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Updated: Jul 1, 2026

In vivo and in vitro Studies of Adaptor-clathrin Interaction
17:14

In vivo and in vitro Studies of Adaptor-clathrin Interaction

Published on: January 26, 2011

Schizosaccharomyces pombe Ddb1 recruits substrate-specific adaptor proteins through a novel protein motif, the

Yasunori Fukumoto1, Naoshi Dohmae, Fumio Hanaoka

  • 1Faculty of Science, Gakushuin University, 1-5-1 Mejiro, Toshima-ku, Tokyo 171-8588, Japan. fumio.hanaoka@gakushuin.ac.jp.

Molecular and Cellular Biology
|September 17, 2008
PubMed

Insights

Researchers discovered a new protein motif, the DDB-box, crucial for DDB1/cullin 4 ubiquitin ligase function. This motif is essential for DNA repair pathways, specifically transcription-coupled nucleotide excision repair.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • DNA Damage Binding Protein 1 (DDB1) is a key component of cullin 4A ubiquitin ligases.
  • Cullin-RING complexes represent a large family of ubiquitin ligases with diverse substrate adaptors.
  • A WDXR motif has been identified as common in DDB1/cullin 4 ubiquitin ligases.

Purpose of the Study:

  • To identify novel protein motifs involved in DDB1/cullin 4 ubiquitin ligase interactions.
  • To investigate the role of these motifs in DNA repair mechanisms.
  • To characterize the function of the DDB-box in vivo.

Main Methods:

  • Protein association studies in Schizosaccharomyces pombe.
  • Identification and characterization of a novel protein motif (DDB-box).
  • Analysis of gene function in transcription-coupled nucleotide excision repair.

Main Results:

  • Schizosaccharomyces pombe Ddb1 interacts with WD40 repeat proteins containing a novel DDB-box motif.
  • The DDB-box is essential for Ddb1 interaction and is independent of WD40 repeats.
  • The DDB-box is critical for the in vivo function of ckn1(+) in DNA repair.

Conclusions:

  • The DDB-box is a novel motif defining adaptor proteins for DDB1/cullin 4 ubiquitin ligases.
  • DDB1 and ckn1(+) are involved in transcription-coupled nucleotide excision repair.
  • The DDB-box plays a vital role in DNA repair processes.

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