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Effects of sodium butyrate on the rearranged c-myc expression in mouse plasmacytoma cells

N Kondoh1, T Oikawa, C Satoh

  • 1Laboratory of Molecular Genetics, Hokkaido University School of Medicine, Sappolo, Japan.

Experimental Cell Research
|September 1, 1991
PubMed

Insights

Sodium butyrate rapidly suppresses c-myc mRNA levels in mouse plasmacytoma cells. This downregulation occurs at the transcriptional level, linked to changes in chromatin structure.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Gene Regulation

Background:

  • c-myc is a proto-oncogene critical for cell proliferation.
  • Mouse plasmacytoma (MPC) cells provide a model for studying gene regulation in cancer.
  • Sodium butyrate is known to affect gene expression and chromatin structure.

Purpose of the Study:

  • To investigate the effect of sodium butyrate on c-myc mRNA expression in MPC cells.
  • To determine the regulatory mechanisms underlying c-myc mRNA suppression.
  • To explore the role of chromatin structure in this process.

Main Methods:

  • Treatment of S194 MPC cells with sodium butyrate.
  • Measurement of steady-state c-myc mRNA levels.
  • Run-on assays to assess transcriptional activity.
  • DNase I sensitivity assays to evaluate chromatin structure.

Main Results:

  • Sodium butyrate treatment suppressed steady-state levels of rearranged c-myc mRNA in S194 cells.
  • Run-on assays confirmed transcriptional downregulation of the c-myc gene.
  • Reduced DNase I sensitivity of the c-myc gene accompanied the suppression.

Conclusions:

  • Sodium butyrate rapidly downregulates c-myc mRNA in mouse plasmacytoma cells.
  • Regulation occurs at the transcriptional level.
  • Changes in DNase I sensitive chromatin structure are involved in this rapid downregulation.

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