Super-resolution imaging in live Caulobacter crescentus cells using photoswitchable EYFP

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Super-resolution Imaging of the Bacterial Division Machinery
08:47

Super-resolution Imaging of the Bacterial Division Machinery

Published on: January 21, 2013

Julie S Biteen1, Michael A Thompson, Nicole K Tselentis

  • 1Department of Chemistry, Stanford University, 375 North-South Mall, Stanford, California 94305, USA.

Nature Methods
|September 17, 2008
PubMed

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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.