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High-sensitivity simultaneous analysis of ubiquinol-10 and ubiquinone-10 in human plasma
Aya Hirayama1, Hiroshi Kubo, Masashi Mita
1Shiseido Research Center, 2-2-1 Hayabuchi, Yokohama 224-8558, Japan.
Abstract:
A method to determine ubiquinol-10 and ubiquinone-10 in human serum was developed by using high-performance liquid chromatography consisting of a semi-microcolumn switching system and an electrochemical detector (ECD), which requires minimized sample pre-treatments. A linear dynamic range was obtained from 1.0 to 5000 ng/mL, and recovery values of 89-105% were observed in a low-concentration region of 10-50 ng/mL. In a long operation test, a good precision was maintained during 5100 runs without any maintenance on ECD or columns. In addition, retention behaviors of other ubiquinone homologues were examined.
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