Related Experiment Video
Updated: Jun 30, 2026

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
Published on: September 2, 2020
Optimization of gradient separation for chromatographic fingerprint of herbal medicine: a predictive model combined
Xiaoying Yin1, Weifeng Yao, Yuzhu Hu
1Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, China.
Abstract:
A four-step strategy is described which is aimed at optimizing the separation parameters of the chromatographic fingerprint of herbal medicines. In the optimization procedure, a model is built to predict the retention time of components. The proposed model shows good retention prediction potency with an average relative deviation of 1.78%. The grid search method is used in optimizing gradient parameters. In addition, an iterative procedure is adopted to further improve the prediction accuracy of the gradient retention time and the quality of the chromatogram. The whole optimization process has been validated by real samples.
Related Concept Videos
Optimizing Chromatographic Separations
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
High-Performance Liquid Chromatography: Elution Process
Principles Of Column Chromatography
Chromatographic Methods: Terminology
Chromatographic Resolution
The effectiveness of separation can be evaluated by determining the level of separation between two neighboring peaks in a chromatogram, which represents the individual components of a sample.
In chromatography,...
Chromatography: Introduction
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
