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Related Concept Videos

Chemotaxis and Direction of Cell Migration01:21

Chemotaxis and Direction of Cell Migration

Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon towards...
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Cytoskeletal Coordination in Cell Migration

A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker proteins that...
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Related Experiment Video

Updated: Jun 30, 2026

Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord
12:23

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Published on: May 25, 2010

Netrin-1 is a survival factor during commissural neuron navigation.

Céline Furne1, Nicolas Rama, Véronique Corset

  • 1Apoptosis, Cancer, and Development Laboratory, Equipe labellisée La Ligue, Centre Léon Berard and Unité Mixte de Recherche 5238, Centre National de la Recherche Scientifique, Université de Lyon, 69008 Lyon, France.

Proceedings of the National Academy of Sciences of the United States of America
|September 18, 2008
PubMed
Summary

Netrin-1 guides developing axons and promotes neuron survival by inhibiting the Deleted in Colorectal Cancer (DCC) protein

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Cancer Biology

Background:

  • Deleted in Colorectal Cancer (DCC) is a tumor suppressor protein.
  • Netrin-1, DCC's ligand, is a survival factor that inhibits DCC's tumor suppressor activity.
  • Netrin-1 is also a key axon guidance cue during spinal cord development.

Purpose of the Study:

  • To investigate the dual role of netrin-1 in neuronal development and cancer.
  • To determine if netrin-1's anti-apoptotic function extends to developing neurons.

Main Methods:

  • Primary neuronal cultures from mice and chick embryos.
  • Analysis of Deleted in Colorectal Cancer (DCC) activity.
  • Assessment of neuronal survival and apoptosis.

Main Results:

  • Netrin-1 acts as an attractive cue for commissural axons.
  • Netrin-1 actively promotes the survival of developing commissural neurons.
  • Netrin-1 inhibits the pro-apoptotic function of DCC in these neurons.

Conclusions:

  • Netrin-1 possesses both attractive and anti-apoptotic functions crucial for commissural axon navigation.
  • Adequate neuronal navigation requires both netrin-1's guidance and anti-apoptotic activities.
  • Netrin-1's dual role highlights its importance in both nervous system development and cancer suppression.