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Updated: Mar 23, 2026

Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues
Published on: October 20, 2019
Cheap, rapid and efficient DNA extraction method to perform multilocus microsatellite genotyping on all Schistosoma
S Beltran1, R Galinier, J F Allienne
1UMR 5244 CNRS-EPHE, Parasitologie Fonctionnelle et Evolutive, Biologie et Ecologie Tropical et Méditerranéene, Université de Perpignan, Perpignan, France.
Abstract:
Schistosomes are endoparasites causing a serious human disease called schistosomiasis. The quantification of parasite genetic diversity is an essential component to understand the schistosomiasis epidemiology and disease transmission patterns. In this paper, we propose a novel assay for a rapid, low costly and efficient DNA extraction method of egg, larval and adult stages of Schistosoma mansoni. One euro makes possible to perform 60,000 DNA extraction reactions at top speed (only 15 min of incubation and 5 handling steps).

