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Updated: Jun 30, 2026
Flat Mount Imaging of Mouse Skin and Its Application to the Analysis of Hair Follicle Patterning and Sensory Axon Morphology
Published on: June 25, 2014
[Transducin beta-TrCP expressed in developmental hair follicle of mice]
Yong-dai Shen1, Yong-hong He, Lin Yang
1State Key Laboratory of Oral Diseases, West China Stomatological Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To identify the expression of antagonist beta-TrCP protein in Sonic hedgehog signal transduction pathway and Wnt signal transduction pathway in hair follicle tissues.
Methods:
The heads of day 18 embryo, and one day and six-days-old postnatal mice were acquired and treated with 40 g/L paraformaldehyde fixation for 48 h and paraffin embedding. The expression of beta-TrCP proteins was examined using LsAB (labelled streptavidin-biotin) method.
Results:
beta-TrCP proteins were expressed in the cytoplasm of the hair stems of hair follicle, hair cuticle, cuticle of root sheath, Huxley's layer of internal root sheath cells, external root sheath and mesenchymal tissues, but not in connective tissue sheath and Henle's layer of internal root sheath.
Conclusion:
TrCP express in the developmental hair follicle tissues, which implicates that beta-TrCP regulate the developmental hair follicle by mediating the signal transduction pathways.
Insights
Beta-transducin repeat-containing protein (beta-TrCP) is expressed in developing hair follicles. This suggests beta-TrCP regulates hair follicle development via signaling pathways.
Area of Science:
- Molecular Biology
- Developmental Biology
- Dermatology
Context:
- The Sonic hedgehog (Shh) and Wnt signaling pathways are crucial for hair follicle development.
- Beta-transducin repeat-containing protein (beta-TrCP) is an antagonist protein involved in these pathways.
- Understanding beta-TrCP expression is key to elucidating hair follicle morphogenesis.
Purpose:
- To investigate the expression pattern of beta-TrCP protein within the hair follicle.
- To determine the role of beta-TrCP in the Sonic hedgehog and Wnt signaling pathways during hair follicle development.
Summary:
- Beta-transducin repeat-containing protein (beta-TrCP) was detected in various hair follicle compartments, including the hair stem, cuticle, and root sheath.
- Expression was observed in mesenchymal tissues but notably absent in the connective tissue sheath and Henle's layer.
- Immunohistochemical analysis using the LsAB method on mouse embryo and postnatal tissues revealed specific localization patterns.
Impact:
- These findings indicate that beta-TrCP plays a regulatory role in hair follicle development.
- The study provides insights into the molecular mechanisms governing hair follicle morphogenesis.
- This research contributes to understanding signaling pathway crosstalk in skin appendage formation.
