Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gas Chromatography: Types of Detectors-II01:19

Gas Chromatography: Types of Detectors-II

In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...
Photoluminescence: Applications01:14

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
Potentiometry: Membrane Electrodes01:15

Potentiometry: Membrane Electrodes

Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at the...
Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development of a New Submaximal Walk Test to Predict Maximal Oxygen Consumption in Healthy Adults.

Sensors (Basel, Switzerland)·2021
Same author

A clinical study of submandibular schwannoma.

Oral surgery, oral medicine, oral pathology and oral radiology·2021
Same author

Effective Cryopreservation of a Bioluminescent Auxotrophic <i>Escherichia coli</i>-Based Amino Acid Array to Enable Long-Term Ready-to-Use Applications.

Biosensors·2021
Same author

Factors for return to emergency department and hospitalization in elderly urinary tract infection patients.

The American journal of emergency medicine·2021
Same author

2020 Korean Consensus Guidelines for Diagnosis and Treatment of Chronic Hand Eczema.

Annals of dermatology·2021
Same author

Age-related differences in revisits to the emergency departments of eight Korean university hospitals.

Archives of gerontology and geriatrics·2021

Related Experiment Video

Updated: Jun 30, 2026

Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors
09:11

Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors

Published on: November 23, 2019

Chemosensors for pyrophosphate.

Sook Kyung Kim1, Dong Hoon Lee, Jong-In Hong

  • 1Division of Nano Science and Department of Chemistry, Ewha Womans University,11-1 Daehyon-Dong, Sodaemun-Ku, Seoul 120-750, Korea.

Accounts of Chemical Research
|September 19, 2008
PubMed
Summary
This summary is machine-generated.

Researchers developed novel fluorescent chemosensors for pyrophosphate (PPi) detection. These sensors utilize metal ion complexes for selective PPi recognition in aqueous solutions, enabling applications in DNA sequencing and cancer research.

More Related Videos

Assaying for Inorganic Polyphosphate in Bacteria
07:20

Assaying for Inorganic Polyphosphate in Bacteria

Published on: January 21, 2019

Bacterial Detection &amp; Identification Using Electrochemical Sensors
09:30

Bacterial Detection & Identification Using Electrochemical Sensors

Published on: April 23, 2013

Related Experiment Videos

Last Updated: Jun 30, 2026

Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors
09:11

Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors

Published on: November 23, 2019

Assaying for Inorganic Polyphosphate in Bacteria
07:20

Assaying for Inorganic Polyphosphate in Bacteria

Published on: January 21, 2019

Bacterial Detection &amp; Identification Using Electrochemical Sensors
09:30

Bacterial Detection & Identification Using Electrochemical Sensors

Published on: April 23, 2013

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Chemical Sensing

Background:

  • Pyrophosphate (PPi) is a critical biomarker in cellular processes, including ATP hydrolysis and DNA replication.
  • Accurate detection of PPi is vital for real-time DNA sequencing and cancer research.
  • Selective anion sensing in aqueous media presents challenges due to strong hydration effects.

Purpose of the Study:

  • To develop and classify chemosensors for the selective detection of pyrophosphate (PPi).
  • To explore PPi sensor designs capable of distinguishing PPi from similar anions like ATP.
  • To demonstrate the bioanalytical application of PPi sensors in post-PCR DNA amplification detection.

Main Methods:

  • Classification of PPi chemosensors based on topological and structural features.
  • Investigation of sensors utilizing metal ion complexes, excimers, displacement approaches, and hydrogen-bonding interactions.
  • Design of receptors to differentiate PPi from ATP based on charge density.

Main Results:

  • Metal ion complexes represent the most successful strategy for PPi chemosensor design.
  • Sensors demonstrated high affinity and selectivity for PPi in 100% aqueous solutions.
  • A PPi metal ion complex sensor was successfully applied for rapid, one-step detection of DNA amplification post-PCR.

Conclusions:

  • Metal ion complex-based chemosensors offer a robust platform for selective PPi detection in aqueous environments.
  • These sensors hold significant potential for advancing real-time DNA sequencing and cancer diagnostics.
  • The developed PPi sensor provides a valuable tool for bioanalytical applications, including monitoring PCR.