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Effect of antibiotics on polymorphonuclear function in iron deficiency anaemia patients & normal volunteers
M Banerjee1, S Kamdar, N A Kshirsagar
1Department of Pharmacology, Seth G.S. Medical College, Bombay.
Abstract:
The effect of erythromycin and gentamicin on polymorphonuclear leukocyte (PMN) functions was assessed in normal individuals and in patients with iron deficiency anaemia (IDA) before and after treatment with iron. The PMN phagocytic function was investigated by the standard method. Erythromycin in vivo significantly increased the PMN phagocytic function from 44.18 +/- 2.08 to 57.0 +/- 1.5 at 8 h and the bactericidal activity from 48.33 +/- 1.97 to 56.7 +/- 0.89 at 8 h in the normal adult male volunteers. A significant increase in phagocytic and bactericidal function of PMNs from IDA patients was also observed after in vivo administration of erythromycin. Gentamicin in vitro reduced the bactericidal activity of PMN from normal volunteers (P less than 0.05) but increased the PMN phagocytic activity in normal volunteers and IDA patients.
Insights
Erythromycin enhances polymorphonuclear leukocyte (PMN) function in healthy individuals and those with iron deficiency anemia (IDA). Gentamicin showed mixed effects on PMN activity in vitro.
Area of Science:
- Immunology
- Pharmacology
- Hematology
Background:
- Polymorphonuclear leukocytes (PMNs) are crucial for innate immunity.
- Iron deficiency anemia (IDA) can impair immune cell function.
- Antibiotics may influence immune responses.
Purpose of the Study:
- To evaluate the impact of erythromycin and gentamicin on PMN functions.
- To compare these effects in healthy individuals and IDA patients.
- To assess changes before and after iron treatment in IDA patients.
Main Methods:
- Assessed PMN phagocytic and bactericidal functions.
- Utilized standard phagocytosis assays.
- Administered erythromycin in vivo and gentamicin in vitro.
Main Results:
- Erythromycin significantly increased PMN phagocytic and bactericidal activity in vivo in both normal and IDA subjects.
- Gentamicin in vitro reduced PMN bactericidal activity in normal volunteers.
- Gentamicin in vitro increased PMN phagocytic activity in normal volunteers and IDA patients.
Conclusions:
- Erythromycin demonstrates a positive effect on PMN functions in vivo.
- Gentamicin exhibits differential in vitro effects on PMN phagocytosis and bacterial killing.
- These findings highlight potential immunomodulatory roles of antibiotics in infection and anemia.