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Related Concept Videos

Source And Potency Of Stem Cells01:27

Source And Potency Of Stem Cells

Stem cells are undifferentiated cells with extensive self-renewal properties that help them maintain their population during the fetal and adult stages of life. They can specialize in all cell types of the human body. However, their differential potential may vary and can be classified into five types. Stem cells can be (1) Totipotent, (2) Pluripotent, (3) Multipotent, (4) Oligopotent, and (5) Unipotent. Each stem cell has a specific origin; the fertilized egg or zygote is a totipotent cell and...
Stem Cell Culture01:17

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
Embryonic Stem Cells00:58

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
Embryonic Stem Cells00:57

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...

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Related Experiment Video

Updated: Jun 30, 2026

Generation of Mice Derived from Induced Pluripotent Stem Cells
11:56

Generation of Mice Derived from Induced Pluripotent Stem Cells

Published on: November 29, 2012

Parthenogenesis-derived multipotent stem cells adapted for tissue engineering applications.

Chester J Koh1, Dawn M Delo, Jang Won Lee

  • 1Wake Forest Institute for Regenerative Medicine, Winston Salem, NC 27157, USA. ckoh@chla.usc.edu

Methods (San Diego, Calif.)
|September 19, 2008
PubMed
Summary
This summary is machine-generated.

Parthenogenetic stem cells, derived from unfertilized eggs, offer a promising alternative for regenerative medicine. These cells avoid ethical concerns associated with embryonic stem cells and show potential in tissue engineering applications.

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Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
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Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

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Comparison of Two Representative Methods for Differentiation of Human Induced Pluripotent Stem Cells into Mesenchymal Stromal Cells
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Comparison of Two Representative Methods for Differentiation of Human Induced Pluripotent Stem Cells into Mesenchymal Stromal Cells

Published on: October 20, 2023

Related Experiment Videos

Last Updated: Jun 30, 2026

Generation of Mice Derived from Induced Pluripotent Stem Cells
11:56

Generation of Mice Derived from Induced Pluripotent Stem Cells

Published on: November 29, 2012

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
09:34

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Published on: November 27, 2017

Comparison of Two Representative Methods for Differentiation of Human Induced Pluripotent Stem Cells into Mesenchymal Stromal Cells
06:24

Comparison of Two Representative Methods for Differentiation of Human Induced Pluripotent Stem Cells into Mesenchymal Stromal Cells

Published on: October 20, 2023

Area of Science:

  • Stem Cell Biology
  • Developmental Biology
  • Regenerative Medicine

Background:

  • Embryonic stem cells (ESCs) are crucial for regenerative medicine but face ethical limitations due to embryo destruction.
  • Parthenogenesis offers a potential alternative source of stem cells, bypassing some ethical concerns.

Purpose of the Study:

  • To detail the isolation and expansion of stem cells from parthenogenetic activation of oocytes.
  • To characterize the stem cell properties and differentiation potential of these parthenogenetic stem cells.

Main Methods:

  • Isolation and expansion of stem cells from activated oocytes.
  • Characterization of stem cell properties through differentiation assays.
  • In vivo assessment of tissue formation and integration.

Main Results:

  • Successfully isolated and expanded parthenogenetic stem cells (PSCs).
  • Demonstrated differentiation into myogenic, osteogenic, adipogenic, and endothelial lineages.
  • Showed in vivo formation of muscle-like and bone-like tissues and integration into injured muscle.

Conclusions:

  • Parthenogenetic stem cells are a viable source of pluripotent cells for tissue engineering.
  • PSCs circumvent ethical issues associated with traditional ESCs.
  • These cells hold significant potential for regenerative medicine applications.