Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Chromosome Replication02:31

Chromosome Replication

Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Post-Transcriptional Size-Dependent Expression of the Fission Yeast Cdc13 Cyclin.

bioRxiv : the preprint server for biology·2026
Same author

Harnessing Higher-Dimensional Fluctuations in an Information Engine.

Physical review letters·2026
Same author

A Tetracycline-Inducible Promoter Replacement System for <i>Schizosaccharomyces pombe</i>.

microPublication biology·2025
Same author

In through the out door: A loop-binding-first model for topological cohesin loading.

BioEssays : news and reviews in molecular, cellular and developmental biology·2024
Same author

Enrichment of rare codons at 5' ends of genes is a spandrel caused by evolutionary sequence turnover and does not improve translation.

eLife·2024
Same author

pomBseen: An automated pipeline for analysis of fission yeast images.

PloS one·2023
Same journal

Csf1 facilitates adaptive membrane lipid remodeling linked to ER-plasma membrane contact sites.

Molecular biology of the cell·2026
Same journal

Differential effects of tropomyosin paralogs on mitochondrial dynamics in <i>Saccharomyces cerevisiae</i>.

Molecular biology of the cell·2026
Same journal

Mutating different α-tubulin acetylation sites has distinct effects on axon terminal morphogenesis in <i>Drosophila melanogaster</i>.

Molecular biology of the cell·2026
Same journal

Novel KIF22 Variants Disrupt Mitosis in Human Chondrocytes and Expand SEMDJL2 Mechanisms.

Molecular biology of the cell·2026
Same journal

Functions and trafficking mechanisms of RIC-8 in <i>C. elegans</i> and mammalian cilia.

Molecular biology of the cell·2026
Same journal

The <i>C. elegans</i> WASH complex supports epithelial polarity by promoting endosomal sorting of E-Cadherin.

Molecular biology of the cell·2026
See all related articles

Related Experiment Video

Updated: Jun 30, 2026

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG
10:11

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG

Published on: July 26, 2024

The Hsk1(Cdc7) replication kinase regulates origin efficiency.

Prasanta K Patel1, Naveen Kommajosyula, Adam Rosebrock

  • 1Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA.

Molecular Biology of the Cell
|September 19, 2008
PubMed
Summary
This summary is machine-generated.

The Hsk1-Dfp1 replication kinase regulates DNA replication origin efficiency in fission yeast. Its concentration controls origin firing, impacting genomic stability and linking chromatin structure to replication timing.

More Related Videos

Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae
08:40

Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae

Published on: October 21, 2022

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Related Experiment Videos

Last Updated: Jun 30, 2026

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG
10:11

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG

Published on: July 26, 2024

Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae
08:40

Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae

Published on: October 21, 2022

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • DNA replication origins initiate DNA synthesis but often fire inefficiently.
  • The mechanisms regulating origin efficiency and its biological significance remain unclear.
  • Origin firing in fission yeast exhibits stochasticity, suggesting a role for a rate-limiting factor.

Purpose of the Study:

  • To investigate the role of the Hsk1-Dfp1 replication kinase in regulating DNA replication origin efficiency.
  • To determine if Hsk1-Dfp1 acts as a diffusible, rate-limiting activator of origin firing.
  • To establish the link between origin efficiency, chromatin structure, and genomic stability.

Main Methods:

  • Manipulating Hsk1-Dfp1 protein levels in fission yeast.
  • Tethering Hsk1-Dfp1 to specific genomic loci.
  • Utilizing photobleaching to assess Hsk1-Dfp1 nuclear diffusion.
  • Analyzing the impact of altered origin firing on genomic instability.

Main Results:

  • Hsk1-Dfp1 levels directly correlate with origin efficiency; higher levels increase efficiency, and lower levels decrease it.
  • Local concentration of Hsk1-Dfp1 near an origin enhances its firing efficiency.
  • Hsk1-Dfp1 is freely diffusible within the nucleus.
  • Altering origin firing rates by manipulating Hsk1-Dfp1 levels leads to increased genomic instability.

Conclusions:

  • Hsk1-Dfp1 acts as a diffusible, rate-limiting activator controlling DNA replication origin efficiency in fission yeast.
  • Origin accessibility to Hsk1-Dfp1 is a key regulatory mechanism, potentially linking chromatin structure to replication timing.
  • Appropriate origin firing efficiency, regulated by Hsk1-Dfp1, is crucial for maintaining genomic stability.