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Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

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Published on: January 20, 2022

Column selection for comprehensive multidimensional ion chromatography.

Robert A Shellie¹, Eadaoin Tyrrell, Christopher A Pohl

¹Australian Centre for Research on Separation Science, School of Chemistry, University of Tasmania, Hobart, Australia.

Journal of Separation Science

|September 23, 2008

Summary

This summary is machine-generated.

This study details selecting ion chromatography (IC) columns for multidimensional IC (IC x IC) separations. A novel method using the linear solvent strength model aids in choosing optimal columns and eluents for faster, more effective anion analysis.

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Area of Science:

Analytical Chemistry
Chromatography

Background:

Comprehensive multidimensional ion chromatography (IC x IC) offers enhanced separation capabilities.
Selecting appropriate columns and optimizing conditions are critical for successful IC x IC method development.

Purpose of the Study:

To establish a systematic approach for selecting ion chromatography (IC) columns for IC x IC applications.
To develop an efficient IC x IC method for separating ten common anions.

Main Methods:

Linear solvent strength model was used to determine a single number for anion selectivity, facilitating column comparison.
Principal component analysis visualized column selectivity differences.
Custom column packing and the use of an eluent suppressor were employed to optimize speed and reduce dilution.
A spreadsheet tool based on the linear solvent strength model aided in selecting second-dimension eluent conditions.

Main Results:

Dionex AS16 and AS20 columns were identified as suitable for the target anion separation.
Custom column lengths were packed to meet speed requirements for both dimensions.
A low flow ratio (<1:20) was achieved, minimizing dilution effects.
The developed IC x IC method successfully separated ten anions.

Conclusions:

The linear solvent strength model provides a valuable tool for IC column selection in IC x IC.
Optimized column selection, custom packing, and eluent conditions enable efficient and rapid anion separations using IC x IC.