Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Glycolysis: Preparatory Phase01:21

Glycolysis: Preparatory Phase

In cellular metabolism (the complete breakdown of glucose to extract energy),  glycolysis is the first step. Glycolysis takes place in the cytoplasm of both prokaryotic and eukaryotic cells. Glucose enters heterotrophic cells in two ways. One method is through secondary active transport, where the transport takes place against the glucose concentration gradient. The other mechanism uses a group of integral proteins called GLUT proteins, also known as glucose transporter proteins. These...
Glycolysis01:23

Glycolysis

Glycolysis, the Embden-Meyerhof pathway, is a central metabolic pathway involved in glucose catabolism. It is highly conserved across most organisms, reflecting its fundamental role in cellular energy production. This process occurs in the cytoplasm and can function both in the presence and absence of oxygen, making it versatile for various organisms and environmental conditions.Stages of GlycolysisGlycolysis is a ten-step pathway that converts glucose into pyruvate, generating a net gain of...
Outcomes of Glycolysis01:13

Outcomes of Glycolysis

Nearly all the energy used by cells comes from the bonds that make up complex organic compounds. These organic compounds are broken down into simpler molecules, such as glucose. As a result, cells extract energy from glucose over many chemical reactions—a process called cellular respiration.
Cellular respiration can occur aerobically (with oxygen) or anaerobically (without oxygen). In the presence of oxygen, cellular respiration starts with glycolysis and continues with pyruvate oxidation, the...
Energy-requiring Steps of Glycolysis01:20

Energy-requiring Steps of Glycolysis

Glucose is the source of nearly all energy used by organisms. The first step of converting glucose into usable energy is called glycolysis. Glycolysis occurs in the cytosol of the cell over two phases: an energy-requiring phase and an energy-releasing phase. Over the first three steps, glucose is converted into different forms and attached to two phosphate groups donated by two ATP molecules, resulting in an unstable sugar. In the next two stages, the unstable sugar splits into two sugar...
Fates of Pyruvate01:20

Fates of Pyruvate

Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
What is Glycolysis?00:56

What is Glycolysis?

Overview
Cells make energy by breaking down macromolecules. Cellular respiration is the biochemical process that converts "food energy" (from the chemical bonds of macromolecules) into chemical energy in the form of adenosine triphosphate (ATP). The first step of this tightly regulated and intricate process is glycolysis. The word glycolysis originates from the Latin glyco (sugar) and lysis (breakdown). Glycolysis serves two main intracellular functions: generating ATP and generating...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Discovery of 4-carboxylate-1,2,3-triazine 1-oxide, their 1,2,3-triazine, and the 3,6-dihydro analogs, as new agents against Chagas diseases and Leishmaniasis.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

An In Silico and In Vitro Approach Identified Potential Trypanothione Synthetase Inhibitors with Trypanocidal Activity.

Molecules (Basel, Switzerland)·2026
Same author

[Aldehyde dehydrogenases as therapeutic targets for oxidative stress management in pathological processes].

Archivos de cardiologia de Mexico·2026
Same author

Fungal pellets as biotechnological tools for addressing environmental and industrial challenges: a comprehensive review.

Critical reviews in biotechnology·2025
Same author

Early Administration of N-Acetylcysteine Provides Renal and Cardiac Mitochondrial and Redox Protection, Preventing the Development of Cardio-Renal Syndrome Type IV Induced by 5/6NX.

Antioxidants (Basel, Switzerland)·2025
Same author

Phytochemical Analysis and Appraisal of Antiproliferative Activity of <i>Magnolia alejandrae</i>.

Metabolites·2025

Related Experiment Video

Updated: Jun 30, 2026

Using Ustilago maydis as a Trojan Horse for In Situ Delivery of Maize Proteins
05:38

Using Ustilago maydis as a Trojan Horse for In Situ Delivery of Maize Proteins

Published on: February 8, 2019

Glycolysis in Ustilago maydis.

Emma Saavedra1, Laura E Ramos-Casillas, Alvaro Marín-Hernández

  • 1Departamento de Bioquímica, Instituto Nacional de Cardiología, México D.F., México.

FEMS Yeast Research
|September 23, 2008
PubMed
Summary

This study quantifies glycolytic enzyme kinetics and fluxes in Ustilago maydis, revealing phosphofructokinase as a key regulatory step. Differences in enzyme activity were observed between yeast and mycelial forms, indicating distinct glycolysis regulation.

More Related Videos

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions
11:42

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions

Published on: September 30, 2016

A Rapid and Efficient Method for Assessing Pathogenicity of Ustilago maydis on Maize and Teosinte Lines
07:09

A Rapid and Efficient Method for Assessing Pathogenicity of Ustilago maydis on Maize and Teosinte Lines

Published on: January 3, 2014

Related Experiment Videos

Last Updated: Jun 30, 2026

Using Ustilago maydis as a Trojan Horse for In Situ Delivery of Maize Proteins
05:38

Using Ustilago maydis as a Trojan Horse for In Situ Delivery of Maize Proteins

Published on: February 8, 2019

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions
11:42

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions

Published on: September 30, 2016

A Rapid and Efficient Method for Assessing Pathogenicity of Ustilago maydis on Maize and Teosinte Lines
07:09

A Rapid and Efficient Method for Assessing Pathogenicity of Ustilago maydis on Maize and Teosinte Lines

Published on: January 3, 2014

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Mycology

Background:

  • Glycolysis is a fundamental metabolic pathway.
  • Ustilago maydis is a significant fungal pathogen.
  • Understanding glycolysis regulation in U. maydis is crucial for its study.

Purpose of the Study:

  • To determine the kinetic parameters of glycolytic enzymes and fluxes in Ustilago maydis.
  • To investigate the impact of growth conditions on enzyme activities.
  • To compare glycolysis regulation between yeast and mycelial forms.

Main Methods:

  • Enzyme activity assays.
  • Determination of V(max) and K(m) values.
  • Analysis of glycolytic fluxes.
  • Comparative analysis of yeast and mycelial forms.

Main Results:

  • Phosphofructokinase activity was identified as a potential flux-controlling step.
  • Aldolase was classified as a class II metalo-aldolase.
  • 3-Phosphoglycerate mutase (PGAM) showed cofactor independence, contrary to predictions.
  • Pyruvate secretion was observed in U. maydis yeast.
  • Mycelial form exhibited significantly higher phosphofructokinase and PGAM activities compared to yeast.

Conclusions:

  • Glycolysis regulation in U. maydis is influenced by growth phase and medium composition.
  • Distinct regulatory mechanisms exist between U. maydis yeast and mycelial forms.
  • This study provides novel insights into fungal glycolysis.