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Simple enrichment and independent cultures to expand bacterial community analysis from Gedongsongo hot spring
Agustina Lulustyaningati Nurul Aminin1, Fida Madayanti Warganegara, Pingkan Aditiawati
1Biochemistry Research Division, Faculty of Mathematics and Natural Science, Institut Teknologi Bandung, Bandung, Indonesia.
Abstract:
Denaturing gradient gel electrophoresis was used to identify bacterial communities from Gedongsongo hot spring, Indonesia, by culture-independent and -dependent procedures. Predominant species were found to be closely related to beta-proteobacteria, particularly from genus Ralstonia and Delftia. Other species present include alpha- and gamma-proteobacteria and Thermus groups. Some of the strains showed unique 16S rDNA sequences.

