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Rapid Assessment of Membrane Protein Quality by Fluorescent Size Exclusion Chromatography
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A gas chromatographic method for quantification of detergents frequently used in membrane protein structural studies

Chaowei Shi1, Wei Shao, Ying Xiong

  • 1National Laboratory for Physical Science at Microscale, School of Life Science, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China.

Analytical Biochemistry
|September 23, 2008
PubMed

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Detergent Purification of Membrane Proteins01:18

Detergent Purification of Membrane Proteins

Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...