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Related Concept Videos

Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

Updated: Jun 30, 2026

Murine Prostate Micro-dissection and Surgical Castration
08:49

Murine Prostate Micro-dissection and Surgical Castration

Published on: May 11, 2016

WNT5A selectively inhibits mouse ventral prostate development.

Sarah Hicks Allgeier1, Tien-Min Lin, Chad M Vezina

  • 1Molecular and Environmental Toxicology Center, University of Wisconsin, Madison, WI 53705, USA.

Developmental Biology
|September 23, 2008
PubMed
Summary

WNT5A plays a crucial role in mouse prostate development by regulating prostatic bud patterning. This signaling molecule restricts ventral prostate growth, impacting overall prostate morphogenesis.

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Modeling Paracrine Noncanonical Wnt Signaling In Vitro
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Last Updated: Jun 30, 2026

Murine Prostate Micro-dissection and Surgical Castration
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Published on: May 11, 2016

A Possible Zebrafish Model of Polycystic Kidney Disease: Knockdown of wnt5a Causes Cysts in Zebrafish Kidneys
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Modeling Paracrine Noncanonical Wnt Signaling In Vitro
11:14

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Published on: December 10, 2021

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Urology

Background:

  • Prostate bud patterning is essential for development but poorly understood.
  • The role of WNT5A in early prostate development requires further investigation.

Purpose of the Study:

  • To investigate the role of WNT5A in the patterning of prostatic buds during fetal mouse urogenital sinus (UGS) development.
  • To elucidate the mechanisms by which WNT5A influences prostate morphogenesis.

Main Methods:

  • Analysis of Wnt5a mRNA expression in the fetal mouse UGS.
  • Phenotypic analysis of Wnt5a null male fetuses.
  • In vitro UGS organ culture with recombinant WNT5A protein.
  • In vivo kidney capsule grafting of WNT5A-treated UGS.
  • In vitro UGS organ culture with WNT5A inhibitory antibody and a known prostatic bud inhibitor.

Main Results:

  • Wnt5a mRNA was focally upregulated in the UGS mesenchyme during prostatic budding.
  • Wnt5a null male fetuses exhibited abnormal UGS morphology and prostatic bud patterns.
  • Recombinant WNT5A protein decreased prostatic bud number in wild-type UGS in vitro.
  • Ventral prostate development was impaired in WNT5A-treated UGS grafts.
  • A WNT5A inhibitory antibody rescued prostatic budding inhibited by 2,3,8,7-tetrachlorodibenzo-p-dioxin.

Conclusions:

  • WNT5A is involved in prostatic bud patterning during mouse development.
  • WNT5A appears to restrict ventral prostate development.
  • Understanding WNT5A's role could offer insights into prostate developmental disorders.