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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Related Experiment Video

Updated: Jun 30, 2026

Bimolecular Fluorescence Complementation
08:54

Bimolecular Fluorescence Complementation

Published on: April 15, 2011

Fluorescence complementation: an emerging tool for biological research.

Y John Shyu1, Chang-Deng Hu

  • 1Department of Medicinal Chemistry and Molecular Pharmacology, Purdue Cancer Center, Purdue University, West Lafayette, IN 47907, USA.

Trends in Biotechnology
|September 23, 2008
PubMed
Summary
This summary is machine-generated.

Fluorescence complementation (FC) visualizes molecular events in living systems using fluorescent proteins. This technology offers insights into biological processes and aids in drug discovery.

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12:42

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation (BiFC-PALM)

Published on: December 22, 2015

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Cell Biology

Background:

  • Fluorescent proteins are vital tools in biological research.
  • Fluorescence complementation (FC) is an emerging application for visualizing molecular events in vivo.
  • Ten fluorescent proteins currently support FC applications.

Purpose of the Study:

  • To review the principles and applications of fluorescence complementation (FC) technologies.
  • To discuss current challenges associated with FC technologies.
  • To examine future prospects for advances in FC.

Main Methods:

  • Review of existing literature on fluorescence complementation.
  • Analysis of FC applications in visualizing molecular events.
  • Discussion of FC's role in drug discovery and biological process insights.

Main Results:

  • FC technologies have been developed to visualize diverse molecular events including protein-protein interactions, post-translational modifications, and mRNA localization.
  • FC has demonstrated utility in drug discovery.
  • FC applications provide significant insights into biological processes.

Conclusions:

  • Fluorescence complementation is a powerful technology for studying molecular events in living cells and organisms.
  • Continued development of FC technologies promises deeper understanding of biological processes and new avenues for drug discovery.
  • Addressing current challenges will enhance the capabilities and applications of FC.