A novel nitrite biosensor based on conductometric electrode modified with cytochrome c nitrite reductase composite...

Area of Science:

• Electrochemistry
• Biosensors
• Enzyme Technology

Background:

• Nitrite is an important water quality parameter.
• Accurate nitrite detection is crucial for environmental monitoring.
• Existing methods for nitrite detection can be complex or time-consuming.

Purpose of the Study:

• To develop a conductometric biosensor for sensitive and rapid nitrite detection.
• To optimize the biosensor's performance using cytochrome c nitrite reductase (ccNiR).
• To evaluate the biosensor's applicability in real water samples.

Main Methods:

• Immobilization of ccNiR from Desulfovibrio desulfuricans on an interdigitated electrode.
• Optimization of biosensor configuration and experimental parameters.
• Conductometric measurements for nitrite quantification.

Main Results:

• The optimized biosensor exhibited a fast response time (10s) and a wide linear range (0.2-120 microM).
• Achieved high sensitivity (0.194 microS/microM) and a low detection limit (0.05 microM).
• Demonstrated good reproducibility, stability over one month, and no significant interference from common ionic species.

Conclusions:

• The developed conductometric biosensor is a promising tool for accurate nitrite monitoring in environmental water.
• The biosensor offers advantages in terms of speed, sensitivity, and ease of use.
• Further applications in water quality assessment are feasible.