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Fluorescent sensor responsive to local viscosity and its application to the imaging of liquid-ordered domain in lipid

Kazuma Yasuhara1, Yoshihiro Sasaki, Jun-ichi Kikuchi

  • 1Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.

Colloids and Surfaces. B, Biointerfaces
|September 23, 2008
PubMed

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Total Internal Reflection Fluorescence Microscopy01:05

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.