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Updated: Jun 30, 2026

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

Quantitative analysis of human tissue-specific differences in methylation.

Jun Igarashi¹, Satomi Muroi, Hiroyuki Kawashima

¹Life Science, Advanced Research Institute for Sciences and Humanities, Nihon University, 12-5, Goban-cho, Chiyoda-ku, Tokyo 102-8251.

Biochemical and Biophysical Research Communications

|September 23, 2008

Summary

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Tissue-specific differentially methylated regions (tDMRs) are conserved between mice and humans. These DNA methylation patterns play a role in tissue function, differentiation, and aging, with variations observed among individuals.

Area of Science:

Epigenetics
Genomics
Developmental Biology

Background:

Tissue-specific differentially methylated regions (tDMRs) are crucial for mammalian development and tissue differentiation.
Understanding the conservation and function of tDMRs across species is essential.

Purpose of the Study:

To quantitatively analyze DNA methylation in human orthologous regions of mouse tDMRs.
To investigate the conservation of tDMRs between mouse and human.
To explore the correlation between DNA methylation levels and spermatocyte counts in human testis.

Main Methods:

Quantitative DNA methylation analysis using Sequenom Mass Array.
Bisulfite treatment of DNA followed by mass spectrometry detection.
Analysis of 13 human orthologous regions and testis DNA from multiple individuals.

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Published on: September 7, 2017

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Last Updated: Jun 30, 2026

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

Targeted DNA Methylation Analysis by Next-generation Sequencing

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

Main Results:

Eight regions confirmed as tDMRs in various human tissues across three individuals.
Evidence of divergent DNA methylation levels among individuals.
Inverse correlation between five testis-specific DMRs and spermatocyte number; positive correlation near TRIM38 and CASZ1 genes.

Conclusions:

tDMRs are conserved between mouse and human.
tDMRs likely regulate tissue function, differentiation, and aging.
Individual variations in DNA methylation levels exist and may correlate with specific cellular processes.