Building consensus spectral libraries for peptide identification in proteomics

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Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
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Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

Henry Lam1, Eric W Deutsch, James S Eddes

  • 1Institute for Systems Biology, Seattle, Washington 98103, USA. kehlam@ust.hk

Nature Methods
|September 23, 2008
PubMed

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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...