Immunolocalization of Aspergillus fumigatus mycelial antigens

Specific immunoglobulin from the sera of patients with antibodies to Aspergillus and from antisera raised in rabbits to Aspergillus fumigatus fractions bound almost exclusively to the mycelial wall, as shown by immunogold labelling of ultra-thin sections. Different layers of the wall were labelled, depending on the source of the antigen used to produce antibody. Internal, cytoplasmic components were generally not labelled, except with antisera raised to crude wall material and to a Concanavalin A (ConA)-binding fraction of a water-soluble preparation.