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Related Experiment Video

Updated: Jun 30, 2026

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

Published on: April 25, 2019

Mesodermal patterning activity of SCL.

Ismail Ismailoglu¹, Gabriel Yeamans, George Q Daley

¹Lillehei Heart Institute and Department of Pediatrics, University of Minnesota, Minneapolis, MN 55455, USA.

Experimental Hematology

|September 24, 2008

Summary

This summary is machine-generated.

The stem cell leukemia (SCL) gene acts as a master regulator, controlling early mesodermal patterning and promoting hematopoietic development. Its expression during critical windows influences cell fate decisions in embryonic development.

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Area of Science:

Developmental Biology
Stem Cell Biology
Hematopoiesis

Background:

The transcription factor SCL is crucial for early hematopoiesis and endothelial development.
Conflicting data exists on whether SCL is a master regulator or supports committed precursors.

Purpose of the Study:

To investigate the precise developmental role of SCL in mesodermal patterning and hematopoietic commitment.
To define the temporal windows of SCL responsiveness during in vitro differentiation.

Main Methods:

Engineered murine embryonic stem cells with a conditional doxycycline-inducible SCL transgene.
Evaluated SCL effects at defined time points during a 6-day in vitro differentiation course.
Utilized chimeric embryoid bodies to assess cell-autonomous effects.

Main Results:

Sustained SCL expression significantly increased hematopoiesis.
Early SCL pulse (48-72 hours) promoted Flk1+ mesoderm at the expense of paraxial mesoderm.
Early SCL also expanded hematopoietic progenitors while repressing paraxial and cardiac markers.

Conclusions:

SCL functions as a master regulator in early mesodermal patterning, influencing cell fate decisions.
These findings complement SCL's known roles in later hematopoietic differentiation.