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Related Experiment Video

Updated: Jun 30, 2026

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Published on: September 16, 2014

High-throughput SPR sensor for food safety.

Marek Piliarik¹, Lucie Párová, Jirí Homola

¹Institute of Photonics and Electronics, Chaberská 57, 182 51 Prague, Czech Republic.

Biosensors & Bioelectronics

|September 24, 2008

Summary

A novel surface plasmon resonance (SPR) biosensor enables rapid, parallel detection of bacterial pathogens. This high-throughput system identifies specific DNA or RNA sequences from pathogens like E. coli in under 15 minutes.

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Published on: October 18, 2010

Area of Science:

Biomedical Engineering
Analytical Chemistry
Microbiology

Background:

Accurate and rapid identification of bacterial pathogens is crucial for effective disease treatment and control.
Existing methods for nucleic acid detection can be time-consuming and may lack high-throughput capabilities.

Purpose of the Study:

To develop and demonstrate a high-throughput surface plasmon resonance (SPR) biosensor for the rapid and parallel detection of specific bacterial pathogen nucleic acids.
To evaluate the sensor's performance in detecting short DNA sequences characteristic of common bacterial pathogens.

Main Methods:

Utilized a high-performance SPR imaging sensor with polarization contrast and internal referencing for high refractive index resolution (2 x 10(-7) RIU).
Microspotted an array of DNA probes specific to bacterial pathogens onto the SPR sensor surface.
Performed nucleic acid detection experiments using target sequences from Brucella abortus, Escherichia coli, and Staphylococcus aureus.

Main Results:

Successfully detected short nucleic acid sequences (20-23 bases) characteristic of bacterial pathogens.
Achieved detection limits as low as 100 pM for specific DNA or RNA sequences.
Demonstrated the capability for parallelized detection of multiple pathogens simultaneously.
Completed detection of specific DNA or RNA sequences in less than 15 minutes.

Conclusions:

The developed SPR biosensor offers a rapid, sensitive, and parallelized platform for identifying bacterial pathogens.
This technology has significant potential for applications in clinical diagnostics, food safety, and environmental monitoring.
The high-throughput nature of the SPR sensor enables faster and more efficient pathogen screening.