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Hair Cells01:22

Hair Cells

Hair cells are the sensory receptors of the auditory system—they transduce mechanical sound waves into electrical energy that the nervous system can understand. Hair cells are located in the organ of Corti within the cochlea of the inner ear, between the basilar and tectorial membranes. The actual sensory receptors are called inner hair cells. The outer hair cells serve other functions, such as sound amplification in the cochlea, and are not discussed in detail here.
The Cochlea01:13

The Cochlea

The cochlea is a coiled structure in the inner ear that contains hair cells—the sensory receptors of the auditory system. Sound waves are transmitted to the cochlea by small bones attached to the eardrum called the ossicles, which vibrate the oval window that leads to the inner ear. This causes fluid in the chambers of the cochlea to move, vibrating the basilar membrane.
Auditory Pathway01:15

Auditory Pathway

Auditory pathways constitute the complex neural circuits responsible for transmitting and interpreting auditory information from the peripheral auditory system to the brain. Sound waves are initially captured by the outer ear, funneled through the ear canal, and reach the tympanic membrane (eardrum). These vibrations are transmitted via the middle ear's ossicles to the inner ear's cochlea.
When viewed cross-sectionally, the cochlea reveals the scala vestibuli and scala tympani flanking the...
Anatomy of the Ear01:16

Anatomy of the Ear

Auditory sensation, commonly called hearing, involves the transformation of sonic waves into neural impulses facilitated by the structures of the auditory organ. The prominent, flesh-like structure on the side of the head, called the auricle, directs sound waves towards the auditory canal. The auricle is often mislabeled as the pinna, a term more aligned with mobile structures like a feline's external ear. The auditory canal penetrates the cranium via the external auditory meatus of the...
The Auditory Ossicles01:11

The Auditory Ossicles

The auditory ossicles of the middle ear transmit sounds from the air as vibrations to the fluid-filled cochlea. The auditory ossicles consist of two malleus (hammer) bones, two incus (anvil) bones, and two stapes (stirrups), one on each side. These bones develop during the fetal stage and are the ones to ossify first. They are fully mature at birth and do not grow afterward.
The aptly named stapes look very much like a stirrup. The three ossicles are unique to mammals, and each plays a role in...
Hearing01:31

Hearing

When we hear a sound, our nervous system is detecting sound waves—pressure waves of mechanical energy traveling through a medium. The frequency of the wave is perceived as pitch, while the amplitude is perceived as loudness.

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Articles linked to this work by shared authors, journal, and citation graph.

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Spontaneous Otoacoustic Emissions in <i>Tecta<sup>Y1870C/+</sup></i> Mice Reflect Changes in Cochlear Amplification and How It Is Controlled by the Tectorial Membrane.

eNeuro·2019
Same author

Increased Spontaneous Otoacoustic Emissions in Mice with a Detached Tectorial Membrane.

Journal of the Association for Research in Otolaryngology : JARO·2015
Same author

Prestin-Dependence of Outer Hair Cell Survival and Partial Rescue of Outer Hair Cell Loss in PrestinV499G/Y501H Knockin Mice.

PloS one·2015
Same author

Loss of the tectorial membrane protein CEACAM16 enhances spontaneous, stimulus-frequency, and transiently evoked otoacoustic emissions.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2014
Same author

Functional regulation of the SLC26-family protein prestin by calcium/calmodulin.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2014
Same author

Marshalin, a microtubule minus-end binding protein, regulates cytoskeletal structure in the organ of Corti.

Biology open·2013
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Related Experiment Video

Updated: Jun 30, 2026

Auditory Brainstem Response and Outer Hair Cell Whole-cell Patch Clamp Recording in Postnatal Rats
09:23

Auditory Brainstem Response and Outer Hair Cell Whole-cell Patch Clamp Recording in Postnatal Rats

Published on: May 24, 2018

Cochlear amplification, outer hair cells and prestin.

Peter Dallos1

  • 1Northwestern University, Departments of Neurobiology and Physiology and Communication Sciences and Disorders, The Hugh Knowles Center, 2240 Campus Drive, Evanston, IL 60208, USA. p-dallos@northwestern.edu

Current Opinion in Neurobiology
|September 24, 2008
PubMed
Summary

Vertebrate auditory organs use mechanical amplification. Mammalian cochleae likely evolved outer hair cells and prestin, a protein enabling motility, to expand frequency range, but the link between these amplification methods remains unclear.

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Working with Auditory HEI-OC1 Cells

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Last Updated: Jun 30, 2026

Auditory Brainstem Response and Outer Hair Cell Whole-cell Patch Clamp Recording in Postnatal Rats
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Investigating Outer Hair Cell Motility with a Combination of External Alternating Electrical Field Stimulation and High-speed Image Analysis
09:35

Investigating Outer Hair Cell Motility with a Combination of External Alternating Electrical Field Stimulation and High-speed Image Analysis

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Working with Auditory HEI-OC1 Cells
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Working with Auditory HEI-OC1 Cells

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Area of Science:

  • Auditory Neuroscience
  • Bioacoustics
  • Cellular Biophysics

Background:

  • Mechanical amplification of sound is crucial for hearing across vertebrates.
  • Non-mammalian vertebrates utilize stereociliary processes for amplification.
  • Mammalian hearing's broad frequency range suggests unique amplification mechanisms.

Purpose of the Study:

  • To investigate the relationship between stereociliary amplification and outer hair cell (OHC) motility in the mammalian cochlea.
  • To understand the role of prestin in OHC function and its contribution to hearing.
  • To elucidate the co-evolution of amplification processes in vertebrate auditory systems.

Main Methods:

  • Analysis of stereociliary processes in non-mammalian vertebrates.
  • Investigation of outer hair cell (OHC) motility and prestin function in mammals.
  • Comparative study of auditory amplification mechanisms across vertebrate classes.

Main Results:

  • Stereociliary processes are linked to mechanotransducer channels and fast adaptation.
  • Outer hair cells (OHCs) are motile cells whose length changes are voltage-driven, powered by prestin.
  • Prestin's role in OHC motility is essential for the extended frequency range of the mammalian cochlea.

Conclusions:

  • The mammalian cochlea likely evolved outer hair cells and prestin for enhanced frequency range.
  • Understanding the interplay between stereociliary and OHC-based amplification is a key challenge in auditory neurobiology.
  • Further research is needed to fully delineate the relationship between these two distinct amplification mechanisms.