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Updated: Jun 30, 2026

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A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms
Published on: October 21, 2010
Rationale for reading fluconazole MICs at 24 hours rather than 48 hours when testing Candida spp. by the CLSI M27-A2
Luis Ostrosky-Zeichner1, John H Rex, Michael A Pfaller
1University of Texas Medical School at Houston, Texas 77030, USA. Luis.Ostrosky-Zeichner@uth.tmc.edu
Antimicrobial Agents and Chemotherapy
|September 24, 2008
Abstract:
We investigated if CLSI M27-A2 Candida species breakpoints for fluconazole MIC are valid when read at 24 h. Analysis of a data set showed good correlation between 48- and 24-h MICs, as well as similar outcomes and pharmacodynamic efficacy parameters, except for isolates in the susceptible dose-dependent category, such as Candida glabrata.

