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Related Experiment Videos

Elemental diet alters macrophage function in mice.

J Shou1, H P Redmond, P Leon

  • 1Harrison Department of Surgical Research, University of Pennsylvania, School of Medicine, Philadelphia 19104.

The Journal of Surgical Research
|September 1, 1991
PubMed
Summary
This summary is machine-generated.

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Elemental diets (ED) can cause bacterial translocation in mice, impairing macrophage antimicrobial functions and reducing TNF-dependent cytotoxicity. This suggests ED may suppress host immunity, possibly due to gut bacteria translocation.

Area of Science:

  • Immunology
  • Gastroenterology
  • Microbiology

Background:

  • Chemically defined liquid elemental diets (ED) induce bacterial translocation to mesenteric lymph nodes (MLN) in animal models.
  • The impact of ED-induced bacterial translocation on host immune function remains largely uncharacterized.

Purpose of the Study:

  • To investigate the effects of ED administration on peritoneal macrophage (PM phi) antimicrobial functions in conventional and endotoxin-resistant mice.
  • To determine if ED-induced changes in PM phi function are linked to bacterial translocation.

Main Methods:

  • Conventional C57/BL6 and endotoxin-resistant C3H/HeJ mice were pair-fed either ED or regular chow (RD) for 14 days.
  • Bacterial cultures were performed on blood, spleen, liver, and MLN.
  • Peritoneal macrophages were assessed for phagocytosis and killing of Candida albicans (CA), superoxide anion (O2-) production, and TNF-dependent cytotoxicity.

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Main Results:

  • ED induced significant bacterial translocation to MLN in conventional mice, but not in endotoxin-resistant mice.
  • Conventional mice on ED showed impaired CA killing by PM phi, reduced O2- production, and decreased TNF-dependent cytotoxicity.
  • PM phi function in endotoxin-resistant mice remained unaffected by ED.

Conclusions:

  • Enteral feeding of ED downregulates host oxidative and antimicrobial mechanisms and TNF-dependent cytotoxicity in conventional mice.
  • These immune alterations may be a consequence of ED-induced bacterial translocation.
  • The findings highlight a potential link between elemental diet composition, gut microbiota, and host immune responses.