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Double immunization strategy with a BoHV-4-vectorialized secreted chimeric peptide BVDV-E2/BoHV-1-gD
G Donofrio1, C Sartori, V Franceschi
1Università di Parma, Facoltà di Medicina Veterinaria, Dipartimento di Salute Animale, Sezione di Malattie Infettive degli Animali, via del Taglio 10, Parma, Italy. gaetano.donofrio@unipr.it
Vaccine
|September 25, 2008
Summary
Researchers developed a novel vaccine vector using a cloned bovine herpesvirus 4 genome. This vector successfully delivered a chimeric peptide, eliciting immune responses in animals, paving the way for polyvalent vaccines.
Area of Science:
- Veterinary Virology
- Vaccine Development
- Molecular Biology
Background:
- Bovine herpesvirus 4 (BoHV-4) is an alphaherpesvirus with potential as a vaccine vector.
- Developing effective polyvalent vaccines against multiple bovine diseases remains a challenge.
Purpose of the Study:
- To engineer a BoHV-4 based vector for delivering a chimeric peptide vaccine.
- To evaluate the expression, secretion, and immunogenicity of the chimeric peptide in vitro and in vivo.
Main Methods:
- Isolation and full genome cloning of BoHV-4.
- Construction of a recombinant BoHV-4 vector expressing a chimeric peptide (bovine viral diarrhea virus E2 ectodomain fused to BoHV-1 gD ectodomain).
- In vitro cell culture and in vivo animal inoculation studies to assess peptide expression, secretion, and immune response.
Main Results:
- The cloned BoHV-4 genome served as a functional vector platform.
- Recombinant virus infected cells robustly expressed and secreted the chimeric peptide.
- Inoculated animals showed successful immune responses to the target antigens (gE2 and gD).
Conclusions:
- A BoHV-4 based vector can be effectively used to deliver chimeric peptide antigens.
- This approach demonstrates potential for developing safe and effective polyvalent vaccines against bovine viral diseases.
- The study highlights the utility of viral vectors in modern vaccine design.
