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In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

Updated: Jun 30, 2026

CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models

CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models

Published on: August 24, 2022

Enhanced gene trapping in mouse embryonic stem cells.

Frank Schnütgen¹, Jens Hansen, Silke De-Zolt

¹Department of Molecular Hematology, University of Frankfurt Medical School, Frankfurt am Main, Germany.

Nucleic Acids Research

|September 25, 2008

Summary

Researchers developed novel gene trap vectors to improve gene trapping efficiency in mouse embryonic stem cells (ESCs). By inducing gene expression, these new vectors overcome limitations of poor endogenous gene expression, aiding in the recovery of under-represented genes.

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Last Updated: Jun 30, 2026

CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models

CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models

Published on: August 24, 2022

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Published on: June 15, 2017

Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange

Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange

Published on: April 27, 2017

Area of Science:

Molecular Biology
Genetics
Developmental Biology

Background:

Gene trapping is a method for generating insertional mutations in mouse embryonic stem cells (ESCs) to study gene function.
Conventional gene trap vectors report gene expression at the insertion site but may under-represent poorly expressed genes in mutant libraries.
Existing gene trap libraries may have limited representation of genes with low endogenous expression levels.

Purpose of the Study:

To develop a novel class of gene trap vectors capable of inducing gene expression at insertion sites.
To overcome the limitation of poor endogenous gene expression in gene trapping.
To enhance the efficiency of gene trapping in high-throughput screens and recover poorly expressed genes.

Main Methods:

Development of new gene trap vectors incorporating the osteopontin enhancer.
Testing the novel vectors in mouse embryonic stem cells (ESCs).
Assessing gene trapping efficiency in high-throughput screening.

Main Results:

Insertion of the osteopontin enhancer significantly increased gene trapping efficiency.
The novel vectors facilitated the recovery of previously under-represented, poorly expressed genes.
Improved identification of disrupted genes through enhanced reporting of gene trap events.

Conclusions:

The developed gene trap vectors effectively induce gene expression, enhancing trapping efficiency.
This approach addresses the under-representation of poorly expressed genes in mutant ESC libraries.
The novel vectors represent a significant advancement for creating comprehensive mutant mouse models.