Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 30, 2026

Study of In Vivo Glucose Metabolism in High-fat Diet-fed Mice Using Oral Glucose Tolerance Test (OGTT) and Insulin Tolerance Test (ITT)

Study of In Vivo Glucose Metabolism in High-fat Diet-fed Mice Using Oral Glucose Tolerance Test (OGTT) and Insulin Tolerance Test (ITT)

Published on: January 7, 2018

Evaluating the glucose tolerance test in mice.

Sofianos Andrikopoulos¹, Amy R Blair, Nadia Deluca

¹Univ. of Melbourne, Dept. of Medicine, Heidelberg Heights, Victoria, Australia. sof@unimelb.edu.au

American Journal of Physiology. Endocrinology and Metabolism

|September 25, 2008

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

2025 ADS/ANZCA/GESA/NACOS clinical practice recommendations on the peri-procedural use of GLP-1/GIP receptor agonists.

Anaesthesia and intensive care·2025

Same author

The management of cardiovascular risk in people with diabetes: Insights from an audit of health services providing diabetes care.

Diabetes research and clinical practice·2025

Same author

Population-level impact of weight loss on predicted healthcare spending and the incidence of obesity-related outcomes in the Asia-Pacific region: a modelling study.

BMC global and public health·2024

Same author

Low hemoglobin, even within the normal range, is associated with diabetic kidney disease.

Diabetes & metabolism·2024

Same author

GPR119 agonists for type 2 diabetes: past failures and future hopes for preclinical and early phase candidates.

Expert opinion on investigational drugs·2024

Same author

Our biology working against us in obesity: A narrative review on implications for management of osteoarthritis.

Osteoarthritis and cartilage open·2023

Optimal glucose tolerance testing in mice involves oral glucose administration after a 6-hour fast. This method best reveals glucose intolerance in high-fat-fed mice compared to chow-fed controls.

Area of Science:

Metabolic research
Animal models
Dietary studies

Background:

High-fat diets induce metabolic changes, impacting glucose homeostasis.
Accurate assessment of glucose tolerance is crucial for metabolic research.

Purpose of the Study:

To identify optimal conditions for assessing glucose tolerance in mice fed standard chow or high-fat diets.
To compare the efficacy of different fasting durations, glucose loads, and administration routes.

Main Methods:

C57BL/6J mice were fed chow or high-fat diets for 8 weeks.
Variables included fasting duration (0-24h, overnight), glucose administration (oral vs. intraperitoneal), glucose dose (2, 1, 0.5 g/kg, fixed 50mg), and state of consciousness.
Glucose tolerance tests (GTTs) were performed under various conditions.

More Related Videos

Intraperitoneal Glucose Tolerance Test, Measurement of Lung Function, and Fixation of the Lung to Study the Impact of Obesity and Impaired Metabolism on Pulmonary Outcomes

Intraperitoneal Glucose Tolerance Test, Measurement of Lung Function, and Fixation of the Lung to Study the Impact of Obesity and Impaired Metabolism on Pulmonary Outcomes

Published on: March 15, 2018

Related Experiment Videos

Last Updated: Jun 30, 2026

Study of In Vivo Glucose Metabolism in High-fat Diet-fed Mice Using Oral Glucose Tolerance Test (OGTT) and Insulin Tolerance Test (ITT)

Study of In Vivo Glucose Metabolism in High-fat Diet-fed Mice Using Oral Glucose Tolerance Test (OGTT) and Insulin Tolerance Test (ITT)

Published on: January 7, 2018

Intraperitoneal Glucose Tolerance Test, Measurement of Lung Function, and Fixation of the Lung to Study the Impact of Obesity and Impaired Metabolism on Pulmonary Outcomes

Intraperitoneal Glucose Tolerance Test, Measurement of Lung Function, and Fixation of the Lung to Study the Impact of Obesity and Impaired Metabolism on Pulmonary Outcomes

Published on: March 15, 2018

Main Results:

A 6-hour fast significantly differentiated glucose tolerance between diet groups (P=0.001).
Oral glucose tolerance tests (OGTTs) showed a greater difference (142%) than intraperitoneal tests (127%).
A glucose dose of 2 g/kg or a fixed 50 mg dose effectively identified glucose intolerance in high-fat-fed mice.

Conclusions:

Oral administration of 2 g/kg glucose after a 6-hour fast is the optimal method for assessing glucose tolerance in mice on chow and high-fat diets.
This protocol effectively detects diet-induced glucose intolerance under conscious or anesthetized conditions.