Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Moderately age-disparate relationships dominate phylogenetically linked pairs involving young women with HIV in KwaZulu-Natal, South Africa.

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases·2026
Same author

Time to change internal medicine training for UK resident doctors.

Lancet (London, England)·2026
Same author

The unexpected role of nurse shark pancreas as a secondary lymphoid organ.

Journal of immunology (Baltimore, Md. : 1950)·2025
Same author

Expanding our concept of simulation in radiology: a "Radiology Requesting" session for undergraduate medical students.

BJR open·2024
Same author

Design and evaluation of the student led ward round - a multidisciplinary intervention allowing practice of essential ward round skills for final year medical students.

Future healthcare journal·2024
Same author

Validity of the UCEEM in use: How Does it Triangulate with Qualitative Data in Measuring the Effect of an Educational Intervention?

Journal of medical education and curricular development·2023
Same journal

Analysis of strength degradation of coal and rock masses and stability of mined areas under long term immersion environment.

PloS one·2026
Same journal

Biogenic Silver-Selenium nanocomposite with anticancer activity and potent efficacy against vancomycin-resistant Staphylococcus aureus.

PloS one·2026
Same journal

Preparation and physicochemical characterization of a biodegradable chitosan/carboxymethyl cellulose hydrogel synthesized in NaOH/urea medium.

PloS one·2026
Same journal

Action-guilt, survivor-guilt, and depression in combat-related PTSD.

PloS one·2026
Same journal

Explainable machine learning for predicting activities of daily living at discharge in stroke patients: A retrospective study using SHAP interpretability.

PloS one·2026
Same journal

Deep learning based two-way feature depiction model for brain tumor detection.

PloS one·2026
See all related articles

Related Experiment Video

Updated: Jun 30, 2026

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
06:01

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

Protein microarray on-demand: a novel protein microarray system.

Deb K Chatterjee1, Kalavathy Sitaraman, Cassio Baptista

  • 1Protein Expression Laboratory, SAIC-Frederick, Inc., NCI-Frederick, Frederick, Maryland, USA. chatterjee@mail.ncifcrf.gov

Plos One
|September 25, 2008
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel DNA microarray that converts to a protein microarray on-demand. This innovative system uses a high-affinity DNA-protein interaction for on-demand protein capture without extra reagents.

More Related Videos

High-throughput Protein Expression Generator Using a Microfluidic Platform
09:26

High-throughput Protein Expression Generator Using a Microfluidic Platform

Published on: August 23, 2012

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
08:07

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Related Experiment Videos

Last Updated: Jun 30, 2026

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
06:01

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

High-throughput Protein Expression Generator Using a Microfluidic Platform
09:26

High-throughput Protein Expression Generator Using a Microfluidic Platform

Published on: August 23, 2012

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
08:07

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Genomics

Background:

  • Protein microarrays are crucial tools in biological research and diagnostics.
  • Current methods for protein microarray fabrication can be complex and costly.
  • There is a need for simpler, more cost-effective protein array generation strategies.

Purpose of the Study:

  • To introduce a novel, low-cost protein microarray strategy.
  • To enable on-demand conversion of DNA microarrays into functional protein arrays.
  • To utilize a specific high-affinity DNA-protein interaction for protein capture.

Main Methods:

  • Printing expression-ready plasmid DNAs encoding fusion proteins onto slides.
  • Utilizing the high-affinity binding between E. coli Tus protein and Ter DNA sequences.
  • Synthesizing proteins of interest as Tus fusion proteins with embedded Ter DNA sequences.
  • Employing the Ter sequence as an integrated capture reagent for the synthesized Tus fusion protein.

Main Results:

  • Demonstrated a "all DNA" microarray system that can be converted to a protein microarray.
  • Achieved on-demand protein capture through a specific DNA-protein interaction.
  • Eliminated the need for additional capture reagents in the protein microarray generation process.

Conclusions:

  • The developed strategy offers a simple, low-cost, and efficient method for generating protein microarrays.
  • This on-demand conversion approach simplifies protein microarray fabrication.
  • The "all DNA" microarray platform holds promise for various applications in biological research and diagnostics.