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Updated: Jun 30, 2026

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer
12:23

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer

Published on: August 2, 2018

Phosphoproteomics, oncogenic signaling and cancer research

Poh-Kuan Chong1, Huiyin Lee, Jacklyn Wai-Fun Kong

  • 1Oncology Research Institute, Yong Loo Lin School of Medicine, Singapore.

Proteomics
|September 25, 2008
PubMed

Insights

Phosphoproteomics advancements have revolutionized cancer research by mapping phosphorylation events and identifying new targets. This technology is key to understanding cancer

Area of Science:

  • Oncology
  • Biochemistry
  • Bioinformatics

Background:

  • Recent years show rapid development in phosphoproteomics techniques.
  • Epidermal growth factor signaling serves as a model system for studying oncogenic signaling pathways.

Purpose of the Study:

  • To review the impact of phosphoproteomics, bioinformatics, and computational modeling on oncogenic signaling.
  • To highlight the role of these technologies in advancing cancer research and therapeutics.

Main Methods:

  • Review of phosphoproteomics methods development over the past 5 years.
  • Application of epidermal growth factor signaling as a model system.
  • Integration of bioinformatics and computational modeling approaches.

Main Results:

  • Phosphoproteomics enables temporal fine mapping of phosphorylation events.
  • Identification of novel tyrosine kinase substrates and phosphorylation sites.
  • Enhanced understanding of cancer's molecular etiology.

Conclusions:

  • Phosphoproteomics is crucial for deciphering complex oncogenic signaling pathways.
  • The next decade will witness significant utilization of phosphoproteomics in cancer research.
  • This technology drives the development of targeted cancer therapeutics.

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