Construction, assembling and application of a trehalase-GOD enzyme electrode system

Area of Science:

• Biochemistry
• Analytical Chemistry
• Food Science

Background:

• Trehalose is a disaccharide crucial in food as a glucose source and preservative.
• Its unique properties make it valuable in food processing, particularly in drying and deep-freezing.
• Accurate determination of trehalose is important for quality control and product development.

Purpose of the Study:

• To develop a novel biosensor for the determination of trehalose.
• To optimize the operational conditions for the biosensor system.
• To demonstrate the biosensor's utility in analyzing real food samples.

Main Methods:

• A flow system biosensor was designed, incorporating a reactor with trehalase enzyme for trehalose hydrolysis.
• A glucose oxidase (GOD) amperometric biosensor was used for quantifying the released glucose.
• Screen-printed electrodes (SPEs) activated with Prussian Blue and modified with Nafion-immobilized GOD were employed.
• Trehalase was chemically immobilized on an Immunodyne ABC membrane within the reactor.

Main Results:

• The study optimized operative conditions for the biosensor.
• Effective immobilization procedures for both trehalase and GOD enzymes were established.
• The biosensor demonstrated successful trehalose determination in a real sample of Boletus edulis mushroom.

Conclusions:

• A functional and sensitive biosensor for trehalose determination has been successfully developed.
• The biosensor system, utilizing enzyme-based hydrolysis and amperometric detection, is effective for food analysis.
• The application in Boletus edulis demonstrates the practical utility of this biosensor in real-world food matrices.