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Translocations involving MUM1 are rare in diffuse large B-cell lymphoma
Kristin E Hunt1, Bryan Hall, Kaaren K Reichard
1Department of Pathology, University of New Mexico, Albuquerque, NM 87102, USA.
Applied Immunohistochemistry & Molecular Morphology : AIMM
|September 26, 2008
Summary
Genetic abnormalities involving MUM1 are rare in diffuse large B-cell lymphoma (DLBCL). Most non-GC DLBCL cases show MUM1 dysregulation through mechanisms other than translocation.
Area of Science:
- Hematology
- Oncology
- Molecular Biology
Background:
- Diffuse large B-cell lymphoma (DLBCL) is a heterogeneous neoplasm.
- DLBCL is classified into germinal center (GC) and non-GC subtypes.
- Non-GC DLBCL often expresses MUM1, suggesting potential MUM1 dysregulation.
Purpose of the Study:
- To investigate the hypothesis that MUM1 is dysregulated by chromosomal translocation in DLBCL.
- To determine the frequency of MUM1 translocations in GC and non-GC DLBCL subtypes.
Main Methods:
- Utilized a novel MUM1 break-apart probe for fluorescence in situ hybridization (FISH).
- Analyzed 33 DLBCL cases (17 GC type, 16 non-GC type) for MUM1 translocations.
- Assessed MUM1 expression via immunohistochemistry in relation to GC/non-GC subtypes.
Main Results:
- Identified one case of MUM1 translocation in 31 evaluable DLBCL cases.
- The identified MUM1 translocation occurred in a non-GC DLBCL subtype (1/15 non-GC cases).
- MUM1 translocation was found to be rare in DLBCL overall.
Conclusions:
- Genetic abnormalities involving MUM1, specifically translocations, are infrequent in DLBCL.
- Mechanisms other than translocation are responsible for MUM1 protein deregulation in the majority of non-GC DLBCL cases.
- Further research is needed to elucidate alternative MUM1 dysregulation pathways in DLBCL.
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