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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
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Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
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Related Experiment Video

Updated: May 7, 2026

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
07:55

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe

Published on: March 7, 2019

Promoter-driven splicing regulation in fission yeast.

Alberto Moldón1, Jordi Malapeira, Natalia Gabrielli

  • 1Oxidative Stress and Cell Cycle Group, Universitat Pompeu Fabra, C/Doctor Aiguader 88, Barcelona 08003, Spain.

Nature
|September 26, 2008
PubMed
Summary

Rem1, a meiosis-specific cyclin in fission yeast, is regulated by transcription and splicing. Mei4 and Fkh2 control its expression, impacting meiotic recombination and cell cycle progression.

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Published on: June 30, 2022

Area of Science:

  • Molecular Biology
  • Cell Cycle Regulation
  • Yeast Genetics

Background:

  • Meiosis differs from mitosis with a pre-meiotic S phase, two divisions, and unique chromosome segregation.
  • Rem1 is a meiosis-specific cyclin in *Schizosaccharomyces pombe* essential for recombination and timely meiosis I onset.
  • Ectopic Rem1 expression causes G1 arrest and mitotic catastrophe in vegetative cells.

Purpose of the Study:

  • To investigate the regulatory mechanisms of *rem1* gene expression at transcriptional and post-transcriptional levels.
  • To elucidate the roles of specific transcription factors in controlling *rem1* splicing and function.

Main Methods:

  • Analysis of *rem1* gene regulation through transcription and splicing.
  • Construction and analysis of chimeric genes with the *rem1* promoter fused to other intron-containing genes.
  • Investigating the involvement of forkhead transcription factors Mei4 and Fkh2.

Main Results:

  • *rem1* expression is controlled by both transcription and alternative splicing, producing distinct proteins.
  • Splicing regulation of *rem1* is independent of its transcribed regions.
  • Mei4 promotes *rem1* transcription and splicing, while Fkh2 mediates intron retention during vegetative growth and pre-meiotic S phase.

Conclusions:

  • *rem1* splicing is meiotic-specific and regulated by Mei4 and Fkh2.
  • Fkh2 plays a dual role in regulating *rem1* splicing, promoting intron retention during specific cell cycle stages.
  • These findings reveal a novel layer of gene regulation critical for meiotic progression in *S. pombe*.