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Updated: Jun 30, 2026

Separation of Spermatogenic Cell Types Using STA-PUT Velocity Sedimentation
Published on: October 9, 2013
Application of a numerical simulation to improve the separation efficiency of a sperm sorter
Toru Hyakutake1, Yuki Hashimoto, Shinichiro Yanase
1Graduate School of Natural Science and Technology, Okayama University, 3-1-1, Tsushimanaka, Okayama, 700-8530, Japan. hyaku@mech.okayama-u.ac.jp
Abstract:
This paper describes a study in which numerical simulations were applied to improve the separation efficiency of a microfluidic-based sperm sorter. Initially, the motion of 31 sperm were modeled as a sinusoidal wave. The modeled sperm were expected to move while vibrating in the fluid within the microchannel. In this analysis, the number of sperm extracted at the outlet channel and the rate of movement of the highly motile sperm were obtained for a wide range of flow velocities within the microchannel. By varying the channel height, and the width and the position of the sperm-inlet channel, we confirmed that the separation efficiency was highly dependent on the fluid velocity within the channel. These results will be valuable for improving the device configuration, and might help to realize further improvements in efficiency in the future.
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