Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
Replication in Prokaryotes02:35

Replication in Prokaryotes

Overview
Replication in Prokaryotes02:35

Replication in Prokaryotes

Overview
Replication in Prokaryotes01:32

Replication in Prokaryotes

DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structural variations in (001)-oriented layered lead halide perovskites, templated by 1,2,4-triazolium.

Dalton transactions (Cambridge, England : 2003)·2020
Same author

Diradical Character of Neutral Heteroleptic Bis(1,2-dithiolene) Metal Complexes: Case Study of [Pd(Me<sub>2</sub>timdt)(mnt)] (Me<sub>2</sub>timdt = 1,3-Dimethyl-2,4,5-trithioxoimidazolidine; mnt<sup>2-</sup> = 1,2-Dicyano-1,2-ethylenedithiolate).

Inorganic chemistry·2020
Same author

Variable dimensionality in 'hollow' hybrid tin iodide perovskites.

Dalton transactions (Cambridge, England : 2003)·2020
Same author

Luminescent Dinuclear Copper(I) Complexes Bearing an Imidazolylpyrimidine Bridging Ligand.

Inorganic chemistry·2020
Same author

NHC-catalyzed enantioselective synthesis of β-trifluoromethyl-β-hydroxyamides.

Beilstein journal of organic chemistry·2020
Same author

Janus Face All-cis 1,2,4,5-tetrakis(trifluoromethyl)- and All-cis 1,2,3,4,5,6-hexakis(trifluoromethyl)- Cyclohexanes.

Angewandte Chemie (International ed. in English)·2020
Same journal

Ir-Catalyzed Formal 1,2-Fluorine Migration in Fluorocarbenes.

Organic letters·2026
Same journal

Leveraging Bayesian Optimization for Accelerated Ligand Selection in Atroposelective Negishi Coupling.

Organic letters·2026
Same journal

Synthesis of Azido Energetic Compounds Based on 2-(Pyrazol-4-yl)pyrazolo[4,3-<i>e</i>][1,2,4]triazolo[1,5-<i>c</i>][1,2,3]triazine via an Atom-Economical Intramolecular Dutt-Wormall-Type Process.

Organic letters·2026
Same journal

Modular Synthesis of Dinitramino-Functionalized Pyrazole-Tetrazole and Its Derivatives: Achieving Superior Detonation Performance.

Organic letters·2026
Same journal

Light-Driven Iron-Catalyzed Decarboxylative Fluorosulfonylation of Aliphatic Carboxylic Acids.

Organic letters·2026
Same journal

Chiral Phosphoric Acid-Catalyzed Enantioselective Dearomative Spirocyclization of Phenol-Ynamides by Kinetic Resolution.

Organic letters·2026
See all related articles

Related Experiment Video

Updated: Jun 30, 2026

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG
10:11

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG

Published on: July 26, 2024

Manipulating replication processes within a dynamic covalent framework.

Vicente del Amo1, Alexandra M Z Slawin, Douglas Philp

  • 1EaStCHEM and Centre for Biomolecular Sciences, School of Chemistry, University of St. Andrews, North Haugh, St. Andrews, Fife KY16 9ST, United Kingdom.

Organic Letters
|September 26, 2008
PubMed
Summary
This summary is machine-generated.

This study demonstrates a dynamic covalent replication cycle where an imine directs its own formation. The resulting amine acts as a superior catalyst for imine synthesis, enhancing the replication process.

More Related Videos

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase
07:37

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase

Published on: September 27, 2024

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
14:56

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

Related Experiment Videos

Last Updated: Jun 30, 2026

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG
10:11

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG

Published on: July 26, 2024

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase
07:37

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase

Published on: September 27, 2024

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
14:56

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

Area of Science:

  • Supramolecular Chemistry
  • Organic Synthesis
  • Chemical Biology

Background:

  • Dynamic covalent chemistry (DCC) enables the formation of reversible covalent bonds, crucial for adaptive materials and molecular self-assembly.
  • Self-replication is a fundamental process in chemistry and biology, allowing for the amplification of specific molecular structures.
  • Molecular recognition elements, such as hydrogen bonding sites, can direct chemical reactions and influence self-assembly.

Purpose of the Study:

  • To investigate the self-replication of an imine through a dynamic covalent cycle.
  • To explore the catalytic role of the imine and its reduced amine product in the replication process.
  • To design molecules with integrated recognition and catalytic functions for efficient self-replication.

Main Methods:

  • Synthesis of an amine bearing an amidopyridine recognition site and an aldehyde bearing a carboxylic acid recognition site.
  • Formation of an imine linkage between the amine and aldehyde components.
  • Investigation of the dynamic covalent replication cycle using spectroscopic and analytical techniques.
  • Reduction of the imine to the corresponding amine and evaluation of its catalytic activity.

Main Results:

  • An imine capable of directing its own formation via a dynamic covalent replication cycle was successfully synthesized.
  • The amidopyridine and carboxylic acid recognition sites effectively directed the imine formation.
  • The amine, derived from the reduction of the replicating imine, demonstrated enhanced catalytic activity compared to the imine itself.
  • This suggests a cascade process where the product amine accelerates the formation of the replicating imine.

Conclusions:

  • The study successfully established a self-replicating imine system driven by dynamic covalent chemistry and molecular recognition.
  • The amine product serves as a more potent catalyst for the imine formation, highlighting a productive autocatalytic loop.
  • This work provides a foundation for designing sophisticated self-replicating systems with potential applications in materials science and synthetic biology.