Related Experiment Video
Updated: Jun 30, 2026

Detection of 3-Nitrotyrosine in Atmospheric Environments via a High-performance Liquid Chromatography-electrochemical Detector System
Published on: January 30, 2019
High-performance liquid chromatography determination of nitrated polycyclic aromatic hydrocarbons by indirect
Salma M Al-Kindy1, James N Miller
1Department of Chemistry College of Science, Sultan Qaboos University PO Box 36, Al-Khod 123, Sultanate of Oman. alkindy@squ.edu.om
Abstract:
A high-performance liquid chromatography (HPLC) method for the analysis of nitrated polcyclic aromatic hydrocarbons (NPAHs) is reported. NPAH mixtures were pre-concentrated using solid-phase extraction and well resolved on a C(18) column. They were detected using an indirect method involving the quenching of the emission from the fluorophores 5,6,7,8-tetrahydronaphthol (5,6,7,8-THN-1-OH), 7-amino-4-methyl coumarin (Coumarin 120, COU-120) and 3-hydroxy-4-(2-hydroxy-4-sulfo-1-naphthylazo)2-naphthalene carboxylic acid (Calcon carboxylic acid, CCA). Linear calibration curves were obtained in the range 1.1 x 10(-9) to 1.1 x 10(-8) mol/L. Using COU 120 as the fluorophore, the detection limit was 2.9 x 10(-10) mol/L for 1-nitronaphthalene and 2.1 x 10(-11) mol/L for 2-nitrofluorene. Recoveries of NPAHs from spiked tap water samples were between 88 and 100%.
More Related Videos
Related Concept Videos
Gas Chromatography: Types of Detectors-II
High-Performance Liquid Chromatography: Types of Detectors
Photoluminescence: Applications
Flame Photometry: Lab
Gas Chromatography: Types of Detectors-I
TCD is the earliest and most widely used detector that operates by measuring the changes in the thermal conductivity of the carrier gas. When a sample compound enters the detector,...
Flame Photometry: Overview

