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[Clostridium difficile: a typing method for epidemiological studies].
G Miscopein1, M Delmée, A Sedallian
1Laboratoire de Bactériologie, Centre Hospitalier Annecy.
Pathologie-Biologie
|May 1, 1991
Summary
This study compared two Clostridium difficile typing methods: polyacrylamide gel electrophoresis (PAGE) and serogrouping. PAGE differentiated 12 profiles, showing good correlation with serogrouping, except for serogroup A.
Area of Science:
- Microbiology
- Clinical Diagnostics
Background:
- Clostridium difficile is a significant cause of antibiotic-associated diarrhea.
- Accurate typing of Clostridium difficile strains is crucial for epidemiological studies and infection control.
Purpose of the Study:
- To evaluate a polyacrylamide gel electrophoresis (PAGE) whole cell protein typing scheme for Clostridium difficile.
- To assess the correlation between the proposed PAGE typing method and the established Delmée serogrouping scheme.
Main Methods:
- Biochemical tests and gas liquid chromatography were used for strain identification.
- Polyacrylamide gel electrophoresis (PAGE) of whole cell proteins was employed for strain typing.
- Comparison with a serogrouping scheme using rabbit antisera.
Main Results:
- A total of 122 Clostridium difficile strains were analyzed.
- PAGE differentiated 12 distinct protein profiles.
- A good correlation was observed between PAGE profiles and serogroups, with notable exceptions in the heterogeneous serogroup A.
Conclusions:
- Polyacrylamide gel electrophoresis (PAGE) offers a reliable method for Clostridium difficile strain typing.
- PAGE provides a valuable complement to serogrouping, enhancing strain differentiation capabilities.