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Updated: Jun 30, 2026
Visual Detection of Multiple Nucleic Acids in a Capillary Array
Published on: November 15, 2017
[DNA polymorphism detection of Papaver somniferum L using fluorescent amplified fragment length polymorphism]
Fan Lu1, Bao-wen Cheng, Hong Li
1Institute of Criminal Science and Technology, Yunnan Province Police Bureau, Kunming 650021, China.
Objective:
To detect DNA polymorphism of Papaver somniferum L using fluorescent Amplified Fragment Length Polymorphism.
Methods:
Genomic DNA was isolated using the AxyPrep DNA Kit, double-digested by two restrictional endonucleases (Eco RI and Mse I) and ligated to oligonucleotide adapters. After Pre-amplification and selective amplification, the DNA fragments were separated by capillary electrophoresis using the CEQ8000 DNA Fragment Analyzer.
Results:
More than 20 fragments of highly polymorphic products were obtained by 8 pairs of primer from 64 selective amplifying primer pairs.
Conclusion:
The fluorescent AFLP technique can be used to detect the DNA polymorphism of Papaver somniferum.
