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Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors
Published on: February 10, 2014
Altered ATP-sensitive potassium channels may underscore obesity-triggered increase in blood pressure
Li-hong Fan1, Hong-yan Tian, Ai-qun Ma
1Department of Cardiology, The First Affiliated Hospital of Xi-an Jiaotong University School of Medicine, Xi-an 710061, China.
Aim:
To determine whether ATP-sensitive potassium channels are altered in VSMC from arotas and mesenteric arteries of obese rat, and their association with obesity-triggered increase in blood pressure.
Methods:
Obesity was induced by 24 weeks of high-fat diet feeding in male Sprague-Dawley rats. Control rats were fed with standard laboratory rat chow. Blood pressure and body weight of these rats were measured every 4 weeks. At the end of 24 weeks, K(ATP) channelmediated relaxation responses in the aortas and mesenteric arteries, K(ATP) channel current, and gene expression were examined, respectively.
Results:
Blood pressure and body weight were increased in rats fed with high-fat diet. K(ATP) channelmediated relaxation responses, currents, and K(ATP) expression in VSMC of both aortas and mesenteric arteries were inhibited in these rats.
Conclusion:
Altered ATP-sensitive potassium channels in obese rats may underscore obesity-triggered increase in blood pressure.
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