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Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper (cTfh) Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry
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Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper (cTfh) Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry

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CD4+ CD28 null T cells in coronary artery disease: when helpers become killers

Ingrid E Dumitriu1, Ernesto Trallero Araguás, Christina Baboonian

  • 1Cardiovascular Biology Research Centre, Division of Cardiac and Vascular Sciences, St George's University of London, Cranmer Terrace, London SW17 0RE, UK.

Cardiovascular Research
|September 27, 2008
PubMed

Insights

CD4(+)CD28(null) T cells, an unusual T cell subset, play a detrimental role in coronary artery disease (CAD). Targeting these cells may offer new therapeutic strategies for preventing cardiovascular events.

Area of Science:

  • Immunology
  • Cardiovascular Medicine
  • Cell Biology

Background:

  • T cells are crucial in atherosclerosis and coronary artery disease (CAD).
  • Helper CD4(+) T cells can influence the atherogenic process, either aggravating or attenuating it.
  • CD4(+)CD28(null) T cells represent an atypical subset of helper T cells that expand in CAD and exert harmful effects.

Purpose of the Study:

  • To review the generation of CD4(+)CD28(null) T cells.
  • To focus on the phenotypic and functional characteristics of CD4(+)CD28(null) T cells relevant to cardiovascular events.
  • To explore the impact of current CAD therapies on CD4(+)CD28(null) T cells.

Main Methods:

  • Literature review of T cell subsets in CAD.
  • Analysis of phenotypic and functional data of CD4(+)CD28(null) T cells.
  • Exploration of therapeutic implications for CAD treatment.

Main Results:

  • CD4(+)CD28(null) T cells are expanded in CAD and contribute to cardiovascular events.
  • These cells possess distinct characteristics that promote atherogenesis.
  • Current CAD therapies may have variable effects on this T cell subset.

Conclusions:

  • CD4(+)CD28(null) T cells are key players in the pathogenesis of CAD.
  • Understanding their role provides insights into disease mechanisms.
  • Targeting CD4(+)CD28(null) T cells presents a potential novel therapeutic strategy for CAD.

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