Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Systemic inflammatory indicators and their clinical correlations in prurigo nodularis: A multicenter cross-sectional study in China.

JAAD international·2026

Same author

Preoperative exercise tolerance, nutritional-inflammatory markers, and outcomes after TEVAR for type B aortic pathology.

Frontiers in cardiovascular medicine·2026

Same author

Effect of exercise interventions on fear of falling in older adults: a systematic review and network meta-analysis of randomised controlled trials.

Age and ageing·2026

Same author

Solvent-Modulated Orthogonal Release from Covalent Organic Frameworks Enables Sequential Multiomics Enrichment.

Journal of the American Chemical Society·2026

Same author

Effect of ropivacaine-dexamethasone combination on postpartum uterine pain, inflammation, and breastfeeding in multiparous women.

American journal of translational research·2026

Same author

Harnessing citizen science to contextualize adaptation mechanism discovery.

Cell·2026

Same journal

Controlled encapsulation and droplet size prediction in two-step microfluidic double emulsions.

Lab on a chip·2026

Same journal

A particulate blood-mimicking fluid with physiological biconcave geometry for microscale hemorheology.

Lab on a chip·2026

Same journal

Multicellular sensor arrays fabricated by capillary stamping for pattern-based odor discrimination.

Lab on a chip·2026

Same journal

A real-time microfluidic surveillance system for multiplex detection of heavy metal contamination in wastewater.

Lab on a chip·2026

Same journal

Vision-guided parallel manipulation of cells with optoelectronic tweezers.

Lab on a chip·2026

Same journal

Review of nanofluidic mass transport systems: engineering through physicochemical fields and interfacial properties.

Lab on a chip·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 30, 2026

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

Cell characterization using a protein-functionalized pore.

Andrea Carbonaro¹, Swomitra K Mohanty, Haiyan Huang

¹Dept. of Mechanical Engineering, University of California at Berkeley, Berkeley, CA 94720-1740, USA.

|September 27, 2008

Summary

This summary is machine-generated.

This study introduces a novel, label-free cell characterization method using artificial pores. It accurately detects specific cell-surface markers by measuring altered current pulse durations, enabling sensitive cell screening.

More Related Videos

Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time

Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time

Published on: March 11, 2021

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Related Experiment Videos

Last Updated: Jun 30, 2026

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time

Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time

Published on: March 11, 2021

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Area of Science:

Biotechnology
Cell Biology
Biosensing

Background:

Cell characterization relies on identifying specific cell-surface markers.
Existing methods often require labels or are less sensitive.
A need exists for rapid, label-free cell analysis techniques.

Purpose of the Study:

To develop and validate a highly sensitive, label-free method for cell characterization.
To utilize artificial pores functionalized with proteins for specific cell-surface receptor detection.
To demonstrate the method's efficacy in screening cell populations based on biomarkers.

Main Methods:

Measuring current pulses generated by individual cells passing through a functionalized artificial pore.
Utilizing specific protein-ligand interactions to retard cell passage and alter pulse duration.
Developing a statistical model for quantitative analysis of mixed cell populations.

Main Results:

Successfully screened murine erythroleukemia cells for the CD34 surface marker.
Demonstrated accurate detection in both single and mixed cell populations.
Achieved high accuracy and sensitivity with small cell numbers (hundreds or less).

Conclusions:

The pore-based method offers a broadly applicable platform for label-free cell characterization.
This technique enables sensitive detection of specific cell-surface biomarkers.
Future applications include a wide range of in vitro cell-based assays.