Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Photoluminescence: Applications01:14

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Photoluminescence: Fluorescence and Phosphorescence01:23

Photoluminescence: Fluorescence and Phosphorescence

Photoluminescence is a process where a molecule absorbs light energy and re-emits it in the form of light. This phenomenon occurs when a substance absorbs photons, promoting its electrons to higher energy level excited states, followed by a relaxation process in which the electrons return to their original ground state energy levels and emit light. Photoluminescence is widely observed in various materials, including semiconductors, and organic and inorganic compounds.
A pair of electrons in a...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Five-year outcomes of transoral robotic surgery with or without neoadjuvant chemotherapy in laryngeal squamous cell carcinoma: a preliminary experience.

Acta otorhinolaryngologica Italica : organo ufficiale della Societa italiana di otorinolaringologia e chirurgia cervico-facciale·2026
Same author

A novel fracture lattice in spiny mouse skin facilitates tissue autotomy and regeneration.

bioRxiv : the preprint server for biology·2026
Same author

Enhancing cultured meat production with ginseng leaf-stem extract: a novel supplementation approach to promote porcine muscle stem cell growth.

Food science of animal resources·2026
Same author

Hypoxia-inducible factors link inflammation and lipid metabolism in atherosclerotic macrophages.

Frontiers in cardiovascular medicine·2026
Same author

Plant Growth Regulators from the Fruiting Bodies and Scrap Cultivation Beds of Hypsizygus marmoreus (Agaricomycetes).

International journal of medicinal mushrooms·2026
Same author

Impact of neoadjuvant chemotherapy on surgical outcomes in patients with HPV-positive oropharyngeal cancer.

Acta oto-laryngologica·2026

Related Experiment Video

Updated: Jun 30, 2026

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
10:21

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

Fluorescent liquid-core/air-cladding waveguides towards integrated optofluidic light sources.

Jong-Min Lim1, Se-Heon Kim, Jae-Hoon Choi

  • 1Department of Chemical and Biomolecular Engineering, KAIST, Daejeon, 305-701, Korea.

Lab on a Chip
|September 27, 2008
PubMed
Summary

We developed novel fluorescent liquid-core/air-cladding (LA) waveguides for optofluidic light sources. These waveguides offer enhanced light confinement and tunable core size, outperforming liquid-core/liquid-cladding designs.

More Related Videos

Lensless Fluorescent Microscopy on a Chip
11:23

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Related Experiment Videos

Last Updated: Jun 30, 2026

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
10:21

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

Lensless Fluorescent Microscopy on a Chip
11:23

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Area of Science:

  • Optofluidics
  • Integrated photonics
  • Materials science

Background:

  • Optofluidic devices require efficient light guiding structures.
  • Existing liquid-core/liquid-cladding waveguides have limitations in optical confinement.

Purpose of the Study:

  • To demonstrate novel fluorescent liquid-core/air-cladding (LA) waveguides.
  • To evaluate their performance as integrated optofluidic light sources.

Main Methods:

  • Fabrication of poly(dimethylsiloxane) (PDMS) channels using soft lithography.
  • Generation of two-phase stratified flows of air and fluorescent dye-infused ethylene glycol.
  • Characterization of optical confinement, propagation losses, and core stream tunability.

Main Results:

  • Achieved strong optical confinement due to high refractive index contrast in LA waveguides.
  • Experimentally determined captured fractions up to 22.8%.
  • Measured low propagation losses of 0.14 dB cm(-1) and demonstrated reversible core size tuning.

Conclusions:

  • LA waveguides are suitable for integrated optofluidic light sources.
  • They offer superior optical confinement and control compared to L(2) waveguides.
  • The absence of diffusional mixing and tunable core size present significant advantages.