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Updated: Jun 30, 2026
Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
Published on: October 4, 2019
Identification of miRNAs associated with tumorigenesis of retinoblastoma by miRNA microarray analysis
Jian-Jun Zhao1, Juhua Yang, Jianhong Lin
1Peking Union Medical College, Tsinghua University, Beijing, China.
Introduction:
MicroRNAs (miRNAs) are small (approximately 22 nucleotides) regulatory RNAs which play fundamental roles in many human diseases, including cancer. There is no report on the miRNA expression profile of retinoblastoma.
Methods:
This work was undertaken to identify differentially expressed miRNAs in human retinoblastoma tissues by microRNA microarray technique, and some miRNAs were verified using northern blot analysis and the in situ hybridization method.
Results:
A cluster of microRNAs was identified as highly expressed in retinoblastoma, including hsa-miR-494, hsa-let-7e, hsa-miR-513-1, hsa-miR-513-2, hsa-miR-518c*, hsa-miR-129-1, hsa-miR-129-2, hsa-miR-198, hsa-miR-492, hsa-miR-498, hsa-miR-320, hsa-miR-503, and hsa-miR-373*.
Conclusion:
These miRNAs are the first to be reported for human retinoblastoma and may play significant roles in regulating tumor genesis.
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