Related Experiment Video
Updated: Jun 30, 2026

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging
Published on: January 5, 2015
Fluorescence studies on the photophysical properties and encapsulation behavior of acetaminophen in different
N S Moyon1, T S Singh, Sivaprasad Mitra
1Department of Chemistry, North-Eastern Hill University, NEHU Permanent Campus, Umshing, Shillong-793022, India.
Abstract:
A systematic study on the spectroscopy and photophysical properties of widely used analgesic and anti-pyretic drug acetaminophen (NAPAP) was presented using steady state and time-resolved fluorescence spectroscopy. The results in homogeneous solvents were compared with those in bio-mimicking environments of cyclodextrin and micellar cavities. Extensive theoretical calculations using time dependent density functional theory (TDDFT) were also done to substantiate the spectral assignment as well as to compare the structure and stability of possible hydrogen bonded conformations of NAPAP in aqueous medium. Facile proton dissociation occurs due to extensive charge redistribution in the excited state. The variation in fluorescence yield and the life time of excited state species in cyclodextrin cavities and micellar medium is due to shift in acid-base dissociation equilibrium in these environments.
More Related Videos
12:07Chemical Modification of the Tryptophan Residue in a Recombinant Ca2+-ATPase N-domain for Studying Tryptophan-ANS FRET
Published on: October 9, 2021
11:28Isolating and Incorporating Light-Harvesting Antennas from Diatom Cyclotella Meneghiniana in Liposomes with Thylakoid Lipids
Published on: August 28, 2018
Related Concept Videos
Variables Affecting Phosphorescence and Fluorescence
Photoluminescence: Applications
Fluorescence and Phosphorescence: Instrumentation
Factors Influencing Drug Absorption: Physicochemical Parameters
Enhanced drug absorption can be achieved by reducing particle sizes and increasing surface areas, thereby facilitating...