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Published on: February 2, 2018
[Establishment of a tight tetracycline-controlled HCV-C double transgenic mouse model]
Li-fang Shuai1, Bo-heng Tang, Ruo-shuang Zhang
1Center for Disease Control and Prevention, Guangzhou, China.
Objective:
To develop a tight tetracycline-controlled HCV-C double transgenic mouse model.
Methods:
By crossbreeding of ApoE-rtTA-tTS transgenic mice with TRE-HCV-C transgenic mice, the double transgenic mice were produced in the F1 generation. The presence of HCV-C and tTS gene in the F1 generation was confirmed by PCR, followed by further identification and quantification of the transgene using Southern blot hybridization. The expression of HCV-C in the liver of the mouse model was detected immunohistochemically.
Results And Conclusion:
Two transgenic mice were obtained, which contained ApoE-rtTA-tTS and TRE-HCV-C genes in the genome. Five founders contained HCV-C gene as confirmed by PCR and Southern blot hybridization. The tight tetracycline-controlled system may facilitate further study of HCV-C gene expression and gene therapy of hepatic cellular carcinoma.
