Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High Intensity Microsecond-Electric Pulses Modulate Cell Death in MDA-MB-231 Cells Treated with Resveratrol.

Cell biochemistry and biophysics·2026
Same author

Key predictive factors of breast cancer based on race using machine learning models.

Annals of epidemiology·2026
Same author

Editorial for the Special Issue, "Artificial Intelligence Applications in Cancer and Other Diseases".

Biomedicines·2026
Same author

Investigations of potential therapeutic targets from high-throughput, label-free, quantitative proteomic studies in MDA-MB-468 cells treated with metformin and electrical pulses.

Bioelectrochemistry (Amsterdam, Netherlands)·2025
Same author

NeuroNet-AD: A Multimodal Deep Learning Framework for Multiclass Alzheimer's Disease Diagnosis.

Bioengineering (Basel, Switzerland)·2025
Same author

Synergistic Enhancement of TNBC Treatment in African American Women: Integrating Resveratrol with Electrical Pulse Therapy.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2025
Same journal

Applications of Recombinant DNA Technology in Medicine: A Comprehensive Review.

Molecular biotechnology·2026
Same journal

Optimizing miRNA Loading into Platelet Exosomes via Electroporation: A Comparative Analysis of Buffer Systems and Voltage Parameters for Enhanced Targeted Drug Delivery.

Molecular biotechnology·2026
Same journal

Regulation of Goat Milk Protein Synthesis: Genetic Architecture, Signalling Pathways, and Omics Insights.

Molecular biotechnology·2026
Same journal

Cloning and Functional Characterization of AhyAP-T65Lig, an ATP-Dependent DNA Ligase from Trabzonvirus AP-T65.

Molecular biotechnology·2026
Same journal

Overexpression of the ATP-Citrate Lyase Gene Enhances Ganoderic Acid Biosynthesis in Ganoderma lingzhi.

Molecular biotechnology·2026
Same journal

CRISPR/Cas9 Mediated Genome Editing for Enhancing Abiotic Stress Tolerance in Rice: An Omics Guided Perspective.

Molecular biotechnology·2026
See all related articles

Related Experiment Video

Updated: Jun 30, 2026

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery
10:51

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

Published on: August 7, 2014

Nanosecond electroporation: another look.

Raji Sundararajan1

  • 1ECET Department, Purdue University, W. Lafayette, IN 47907, USA. rajis@ieee.org

Molecular Biotechnology
|September 30, 2008
PubMed
Summary
This summary is machine-generated.

Nanosecond electroporation (nsEP) offers a safe, non-viral method for gene delivery. This technique uses high-intensity, short electrical pulses for efficient DNA transfer, advancing gene therapy and potentially drug/vaccine delivery.

More Related Videos

Porous Substrate-Based Electroporation with Transepithelial Electrical Impedance Monitoring
08:06

Porous Substrate-Based Electroporation with Transepithelial Electrical Impedance Monitoring

Published on: September 27, 2024

Monitoring Electroporation-Induced Changes in Action Potential Generation in Genetically Engineered Tet-On Spiking HEK cells
10:12

Monitoring Electroporation-Induced Changes in Action Potential Generation in Genetically Engineered Tet-On Spiking HEK cells

Published on: September 6, 2024

Related Experiment Videos

Last Updated: Jun 30, 2026

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery
10:51

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

Published on: August 7, 2014

Porous Substrate-Based Electroporation with Transepithelial Electrical Impedance Monitoring
08:06

Porous Substrate-Based Electroporation with Transepithelial Electrical Impedance Monitoring

Published on: September 27, 2024

Monitoring Electroporation-Induced Changes in Action Potential Generation in Genetically Engineered Tet-On Spiking HEK cells
10:12

Monitoring Electroporation-Induced Changes in Action Potential Generation in Genetically Engineered Tet-On Spiking HEK cells

Published on: September 6, 2024

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Medical Technology

Background:

  • The shift towards gene therapy necessitates advanced, safe, and efficient gene delivery systems.
  • Electroporation, a non-viral method, is a promising technique for gene delivery, with potential applications in drug and vaccine delivery.
  • Traditional electroporation uses millisecond or microsecond pulses.

Purpose of the Study:

  • To review the recent advancements and applications of high-intensity nanosecond electrical pulses for gene delivery.
  • To explore the potential of nanosecond electroporation (nsEP) as a novel gene delivery system.

Main Methods:

  • Review of existing literature on nanosecond electroporation (nsEP) for gene delivery.
  • Analysis of studies utilizing high-intensity nanosecond electrical pulses (10-300 ns) at high magnitudes (10-300 kV/cm).

Main Results:

  • Nanosecond electroporation (nsEP) has shown promise for direct DNA transfer to the nucleus in vitro.
  • High-intensity nanosecond pulses represent a novel approach in electroporation-based gene delivery systems.

Conclusions:

  • Nanosecond electroporation (nsEP) is an emerging and efficient technique for gene delivery.
  • This method holds potential for broader applications in drug and vaccine delivery, complementing traditional gene therapy approaches.