Isolation and molecular identification of extracellular polymeric substances (EPS) producing bacterial strains for...

One of the major problems in overall wastewater treatment process is sludge settling and dewatering. In general, sludge settling and dewatering is carried out using conventional physico-chemical methods that are known to be expensive, and these processes further increase the sludge volume and ultimate disposal costs. To overcome this problem, a suitable alternative could be the use of bioflocculants for sludge settling and dewatering. To achieve bioflocculation, extracellular polymeric substances (EPS) producing bacterial strains were isolated from the complex microbial community of wastewater sludge. Crude EPS produced in the form of bacterial broth was used to test kaolin flocculation activity. Three out of 10 bacterial strains (B2, B8 and B9) were pre-selected for sludge settling. Based on sludge settling and dewatering results, B8 possessed better flocculating property than other bacterial strains. These sludge microorganisms were identified based on their 16S rDNA sequences and bacterial strain B8 was identified as Serratia sps.